In vitro behavior of layer-by-layer deposited molecular oligoelectrolyte films on Ti-6Al-4V surfaces

Journal article
(Original article)


Publication Details

Author(s): Ponader S, Rosenlehner K, Vairaktaris E, von Wilmowsky C, Schlegel KA, Neukam FW, Schmidt C, Schunk T, Hirsch A, Nkenke E
Journal: Journal of Materials Science-Materials in Medicine
Publisher: Springer Verlag (Germany)
Publication year: 2009
Volume: 20
Journal issue: 12
Pages range: 2455-2463
ISSN: 0957-4530


Abstract


Layer-by-layer self-assembled films of molecular oligoelectrolytes were used to modify Ti-6Al-4V surfaces in order to test their ability as potential drug delivery system. With regard to medical application the in vitro behavior of the modified material was investigated. The Ti-6Al-4V (6% aluminium, 4% vanadium) material was treated in a layer-by-layer (LbL) process with 2, 4, 6 and 8 layers of molecular oligoelectrolytes 1 and 2 and thereby doped with a fluorescent reporter molecule 2. Human osteoblasts were cultured for a period up to 5 days on the modified material. Ti-6Al-4V surfaces without modification were used as control. In order to investigate the in vitro behavior of the coating as well as the influence of components of the coating on osteoblastic cells, respectively, cell proliferation, differentiation and attachment of hFOB cells were observed by means of cell number, osteoblastic gene expression and fluorescence microscopy. Degradation behavior of the OEM (oligoelectrolyte multilayer film) was examined using optical spectroscopy. Measurement data imply that the layer-by-layer coating was successfully assembled on the Ti surface and endures steam sterilization. The fluorescence signal in cell culture medium increased strictly linear with increasing pre-assembled number of layers on the surface. Proliferation rates of the cells in experimental groups did not differ significantly from each other (P ≥ 0.783). Differentiation pattern was not significantly changed by the coating. The fluorescent reporter component of the film was absorbed by osteoblastic cells and was detected by fluorescence microscopy. © 2009 Springer Science+Business Media, LLC.



FAU Authors / FAU Editors

Hirsch, Andreas Prof. Dr.
Lehrstuhl für Organische Chemie II
Neukam, Friedrich Wilhelm Prof. Dr. Dr.
Lehrstuhl für Zahnmedizin, insbesondere Mund-, Kiefer-, und Gesichtschirurgie
Department Chemie und Pharmazie
von Wilmowsky, Cornelius PD Dr.
Mund-, Kiefer- und Gesichtschirurgische Klinik


External institutions with authors

National and Kapodistrian University of Athens


How to cite

APA:
Ponader, S., Rosenlehner, K., Vairaktaris, E., von Wilmowsky, C., Schlegel, K.A., Neukam, F.W.,... Nkenke, E. (2009). In vitro behavior of layer-by-layer deposited molecular oligoelectrolyte films on Ti-6Al-4V surfaces. Journal of Materials Science-Materials in Medicine, 20(12), 2455-2463. https://dx.doi.org/10.1007/s10856-009-3825-y

MLA:
Ponader, Sabine, et al. "In vitro behavior of layer-by-layer deposited molecular oligoelectrolyte films on Ti-6Al-4V surfaces." Journal of Materials Science-Materials in Medicine 20.12 (2009): 2455-2463.

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Last updated on 2019-15-04 at 21:50

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