Comparative studies on manganese-based SOD mimetics, including the phosphate effect, by using global spectral analysis

Journal article
(Original article)


Publication Details

Author(s): Friedel F, Lieb D, Ivanovic-Burmazovic I
Journal: Journal of Inorganic Biochemistry
Publisher: Elsevier
Publication year: 2012
Volume: 109
Journal issue: null
Pages range: 26-32
ISSN: 0162-0134


Abstract


Stopped-flow measurements have been successfully combined with time-resolved UV/vis spectroscopy and global spectral analysis of superoxide decay for the comparative study of catalytic activity of putative manganese SOD (superoxide dismutase) mimetics known in the literature. The SOD activity of the studied complexes decreases in the following order: a) M40403 > Mn(III)TM-4-PyP > Mn(II)pyane > > EUK-113 at pH 7.4 (M40403 = ([manganese(II)dichlorido{(4R,9R,14R,19R)-3,10,13,20,26- pentaazatetracyclo[20.3.1.0.4,9014,19]hexacosa-1(26),-22(23),24-triene}], Mn(III)TM-4-PyP = Mn(III)meso-tetrakis(N-methylpyridinium-4-yl) porphyrin, Mn(II)Pyane = Mn(II)trans-2,13-dimethyl-3,6,9,12,18- pentaazabicyclo[12.3.1]-octadeca-1(18),14,16-triene, EUK-113 = [manganese(III)acetato(6,6′-((1E,1′E)-(ethane-1,2- diylbis(azanylylidene))bis(methanylylidene))bis(2-methoxyphenolate)]) in Hepes buffer (Hepes = 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), b) Mn(III)TM-4-PyP > M40403 ≅ Mn(II)pyane ≫ EUK-113 at pH 8.1 in Hepes buffer and c) Mn(III)TM-4-PyP ≅ Mn(II)pyane > MnCl > M40403 ≅ EUK-113 at pH 7.4 in phosphate buffer. MnCl in Hepes, Mn(III)TSPP , Mn(III)TBAP , Mn(III)acetate, Mn(II)lactate and Mn(II)EDTA do not exhibit SOD activity (Mn(III)TSPP = Mn(III)mesotetrakis(benzoic acid)porphyrin, Mn(III)TBAP = Mn(III)mesotetrakis(4-sulfonatophenyl)porphyrin). MnCl possess SOD activity that is comparable with those of other mimetics in the presence of phosphate. Our results demonstrate that phosphate anions, independent of ionic strength effect, influence the activity of manganese SOD mimetics, which is crucial for understanding and comparing their biological activity. © 2011 Elsevier Inc. All rights reserved.



FAU Authors / FAU Editors

Friedel, Felix
Sonderforschungsbereich 583 Redoxative Metallkomplexe - Reaktivitätssteuerung durch molekulare Architekturen (Sprecher: Prof.Dr.Kisch)
Ivanovic-Burmazovic, Ivana Prof. Dr.
Lehrstuhl für Bioanorganische Chemie
Lieb, Dominik Dr.
Lehrstuhl für Bioanorganische Chemie


How to cite

APA:
Friedel, F., Lieb, D., & Ivanovic-Burmazovic, I. (2012). Comparative studies on manganese-based SOD mimetics, including the phosphate effect, by using global spectral analysis. Journal of Inorganic Biochemistry, 109(null), 26-32. https://dx.doi.org/10.1016/j.jinorgbio.2011.12.008

MLA:
Friedel, Felix, Dominik Lieb, and Ivana Ivanovic-Burmazovic. "Comparative studies on manganese-based SOD mimetics, including the phosphate effect, by using global spectral analysis." Journal of Inorganic Biochemistry 109.null (2012): 26-32.

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Last updated on 2019-10-04 at 21:50