Qualitative Determination of Specific Protein Glycation Products by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Peptide Mapping

Journal article


Publication Details

Author(s): Humeny A, Kislinger T, Becker CM, Pischetsrieder M
Journal: Journal of Agricultural and Food Chemistry
Publisher: American Chemical Society
Publication year: 2002
Volume: 50
Pages range: 2153-2160
ISSN: 0021-8561


Abstract


The nonenzymatic reaction between reducing sugars and proteins, known as the Maillard reaction, has received increased recognition from nutritional science and medical research. The development of new analytical techniques for the detection of protein-bound Maillard products is therefore crucial. In this study, we applied peptide mapping by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to investigate the formation of structurally specific Maillard products on glycated lysozyme (AGE-lysozyme), produced upon incubation with D-glucose. In parallel, we synthesized Nε-(carboxymethyl)lysine-modified lysozyme (CML-lysozyme) and Nε-(carboxyethyl)lysine-modified lysozyme, two well-described glycation products, as model substances. 3-Deoxyglucosone-modified lysozyme and methylglyoxal-modified lysozyme were prepared as examples of glycation products incubated with dicarbonyl compounds. We were able to detect specific modifications on AGE-lysozyme, which were assigned to CML, imidazolone A, and the Amadori product.



FAU Authors / FAU Editors

Becker, Cord-Michael Prof. Dr.
Lehrstuhl für Biochemie und Molekulare Medizin
Humeny, Andreas Prof. Dr.
Medizinische Fakultät
Pischetsrieder, Monika Prof. Dr.
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)


Additional Organisation
Emil-Fischer-Zentrum (Emil Fischer Center)


How to cite

APA:
Humeny, A., Kislinger, T., Becker, C.-M., & Pischetsrieder, M. (2002). Qualitative Determination of Specific Protein Glycation Products by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Peptide Mapping. Journal of Agricultural and Food Chemistry, 50, 2153-2160. https://dx.doi.org/10.1021/jf011349o

MLA:
Humeny, Andreas, et al. "Qualitative Determination of Specific Protein Glycation Products by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Peptide Mapping." Journal of Agricultural and Food Chemistry 50 (2002): 2153-2160.

BibTeX: 

Last updated on 2018-19-04 at 02:39