The microprocessor component, DGCR8, is essential for early B-cell development in mice

Journal article


Publication Details

Author(s): Brandl A, Daum P, Brenner S, Schulz S, Yap DYH, Boesl MR, Wittmann J, Schuh W, Jäck HM
Journal: European Journal of Immunology
Publication year: 2016
Volume: 46
Journal issue: 12
Pages range: 2710-2718
ISSN: 0014-2980


Abstract


microRNAs (miRNAs) are important posttranscriptional regulators during hematopoietic lineage commitment and lymphocyte development. Mature miRNAs are processed from primary miRNA transcripts in two steps by the microprocessor complex, consisting of Drosha and its partner DiGeorge Critical Region 8 (DGCR8), and the RNAse III enzyme, Dicer. Conditional ablations of Drosha and Dicer have established the importance of both RNAses in B- and T-cell development. Here, we show that a cre-mediated B-cell specific deletion of DGCR8 in mice results in a nearly complete maturation block at the transition from the pro-B to the pre-B cell stage, and a failure to upregulate Ig ? heavy chain expression in pro-B cells. Furthermore, we found that the death of freshly isolated DGCR8-deficient pro-B cells could be partially prevented by enforced Bcl2 expression. We conclude from these findings that the microprocessor component DGCR8 is essential for survival and differentiation of early B-cell progenitors.



FAU Authors / FAU Editors

Daum, Patrick
Molekular-Immunologische Abteilung in der Medizinischen Klinik 3
Jäck, Hans-Martin Prof. Dr.
Molekular-Immunologische Abteilung in der Medizinischen Klinik 3
Schuh, Wolfgang Dr. rer. nat.
Molekular-Immunologische Abteilung in der Medizinischen Klinik 3
Schulz, Sebastian
Professur für Immunologie
Wittmann, Jürgen Dr. rer. nat.
Molekular-Immunologische Abteilung in der Medizinischen Klinik 3
Yap, Desmond Yat Hin Dr.
Klinisch-Molekularbiologisches Forschungszentrum


External institutions with authors

Universitätsklinikum Würzburg


How to cite

APA:
Brandl, A., Daum, P., Brenner, S., Schulz, S., Yap, D.Y.H., Boesl, M.R.,... Jäck, H.-M. (2016). The microprocessor component, DGCR8, is essential for early B-cell development in mice. European Journal of Immunology, 46(12), 2710-2718. https://dx.doi.org/10.1002/eji.201646348

MLA:
Brandl, Andreas, et al. "The microprocessor component, DGCR8, is essential for early B-cell development in mice." European Journal of Immunology 46.12 (2016): 2710-2718.

BibTeX: 

Last updated on 2018-21-11 at 06:02