The microprocessor component, DGCR8, is essential for early B-cell development in mice

Brandl A, Daum P, Brenner S, Schulz S, Yap DYH, Boesl MR, Wittmann J, Schuh W, Jäck HM (2016)

Publication Type: Journal article

Publication year: 2016

Journal

European Journal of Immunology Wiley-VCH Verlag

Book Volume: 46

Pages Range: 2710-2718

Journal Issue: 12

DOI: 10.1002/eji.201646348

Abstract

microRNAs (miRNAs) are important posttranscriptional regulators during hematopoietic lineage commitment and lymphocyte development. Mature miRNAs are processed from primary miRNA transcripts in two steps by the microprocessor complex, consisting of Drosha and its partner DiGeorge Critical Region 8 (DGCR8), and the RNAse III enzyme, Dicer. Conditional ablations of Drosha and Dicer have established the importance of both RNAses in B- and T-cell development. Here, we show that a cre-mediated B-cell specific deletion of DGCR8 in mice results in a nearly complete maturation block at the transition from the pro-B to the pre-B cell stage, and a failure to upregulate Ig ? heavy chain expression in pro-B cells. Furthermore, we found that the death of freshly isolated DGCR8-deficient pro-B cells could be partially prevented by enforced Bcl2 expression. We conclude from these findings that the microprocessor component DGCR8 is essential for survival and differentiation of early B-cell progenitors.

Authors with CRIS profile

Patrick Daum Department of Molecular Immunology Sebastian Schulz Professur für Immunologie Desmond Yat Hin Yap Klinisch-Molekularbiologisches Forschungszentrum Jürgen Wittmann Department of Molecular Immunology Wolfgang Schuh Department of Molecular Immunology Hans-Martin Jäck Department of Molecular Immunology

Involved external institutions

Universitätsklinikum Würzburg DE Germany (DE)

How to cite

APA:

Brandl, A., Daum, P., Brenner, S., Schulz, S., Yap, D.Y.H., Boesl, M.R.,... Jäck, H.-M. (2016). The microprocessor component, DGCR8, is essential for early B-cell development in mice. European Journal of Immunology, 46(12), 2710-2718. https://dx.doi.org/10.1002/eji.201646348

MLA:

Brandl, Andreas, et al. "The microprocessor component, DGCR8, is essential for early B-cell development in mice." European Journal of Immunology 46.12 (2016): 2710-2718.

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