Glomerulocapillary miRNA response to HLA-class I antibody in vitro and in vivo

Heinemann FM, Jindra PT, Bockmeyer CL, Zeuschner P, Wittig J, Hoeflich H, Esser M, Abbas M, Dieplinger G, Stolle K, Vester U, Hoyer PF, Immenschuh S, Heinold A, Horn PA, Li W, Eisenberger U, Becker JU (2017)


Publication Type: Journal article

Publication year: 2017

Journal

Book Volume: 7

Pages Range: 14554

Journal Issue: 1

DOI: 10.1038/s41598-017-14674-5

Abstract

Changes in miRNA expression glomerular of capillaries during antibody-mediated rejection (ABMR) are poorly understood and could contribute to the deleterious inflammation and fibrosis of ABMR via suppression of target genes. A better understanding could lead to novel diagnostic tools and reveal novel therapeutic targets. We explored deregulated miRNAs in an glomeruloendothelial in vitro model of ABMR due to class I human leukocyte antigen (HLA) with and without complement activation. We studied a set of 16 promising candidate miRNAs in microdissected glomeruli a confirmation set of 20 human transplant biopsies (DSA+) compared to 10 matched controls without evidence for ABMR. Twelve out of these 16 glomerulocapillary miRNAs could successfully be confirmed as dysregulated in vivo with 10 upregulated (let-7c-5p, miR-28-3p, miR-30d-5p, miR-99b-5p, miR-125a-5p, miR-195-5p, miR-374b-3p, miR-484, miR-501-3p, miR-520e) and 2 downregulated (miR29b-3p, miR-885-5p) in DSA+ vs.A random forest analysis based on glomerular miRNAs identified 18/20 DSA+ and 8/10 controls correctly. This glomerulocapillary miRNA signature associated with HLA class I-DSA could improve our understanding of ABMR and be useful for diagnostic or therapeutic purposes.

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How to cite

APA:

Heinemann, F.M., Jindra, P.T., Bockmeyer, C.L., Zeuschner, P., Wittig, J., Hoeflich, H.,... Becker, J.U. (2017). Glomerulocapillary miRNA response to HLA-class I antibody in vitro and in vivo. Scientific Reports, 7(1), 14554. https://dx.doi.org/10.1038/s41598-017-14674-5

MLA:

Heinemann, Falko M., et al. "Glomerulocapillary miRNA response to HLA-class I antibody in vitro and in vivo." Scientific Reports 7.1 (2017): 14554.

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