Benzo[a]pyrene-induced metabolic shift from glycolysis to pentose phosphate pathway in the human bladder cancer cell line RT4

Journal article


Publication Details

Author(s): Verma N, Pink M, Boland S, Rettenmeier AW, Schmitz-Spanke S
Journal: Scientific Reports
Publication year: 2017
Volume: 7
Journal issue: 1
Pages range: 9773
ISSN: 2045-2322


Abstract

Benzo[a]pyrene (B[a]P), a well-known polyaromatic hydrocarbon, is known for its lung carcinogenicity, however, its role in bladder cancer development is still discussed. Comparative two-dimensional blue native SDS-PAGE analysis of protein complexes isolated from subcellular fractions of 0.5 µM B[a]P-exposed cells indicated a differential regulation of proteins involved in carbohydrate, fatty acid, and nucleotide metabolism, suggesting a possible metabolic flux redistribution. It appeared that B[a]P exposure led to a repression of enzymes (fructose-bisphosphate aldolase A, glucose-6-phosphate isomerase, lactate dehydrogenase) involved in glycolysis, and an up-regulation of proteins (glucose-6-phosphate 1-dehydrogenase, 6-phosphogluconolactonase) catalyzing the pentose phosphate pathway and one carbon metabolism (10-formyltetrahydrofolate dehydrogenase, bifunctional purine biosynthesis protein). Untargeted metabolomics further supported the proteomic data, a lower concentration of glycolytic metabolite was observed as compared to glutamine, xylulose and fatty acids. The analysis of the glutathione and NADPH/NADP+ content of the cells revealed a significant increase of these cofactors. Concomitantly, we did not observe any detectable increase in the production of ROS. With the present work, we shed light on an early phase of the metabolic stress response in which the urothelial cells are capable of counteracting oxidative stress by redirecting the metabolic flux from glycolysis to pentose phosphate pathway.


FAU Authors / FAU Editors

Pink, Mario Dr.
Lehrstuhl für Arbeits- und Sozialmedizin
Schmitz-Spanke, Simone Prof. Dr.
Professur für Biomarker in der Arbeitsmedizin
Verma, Nisha Dr.
Professur für Biomarker in der Arbeitsmedizin


Additional Organisation
Exzellenz-Cluster Engineering of Advanced Materials


External institutions with authors

Universitätsklinikum Essen


Research Fields

Nanosafety
Exzellenz-Cluster Engineering of Advanced Materials


How to cite

APA:
Verma, N., Pink, M., Boland, S., Rettenmeier, A.W., & Schmitz-Spanke, S. (2017). Benzo[a]pyrene-induced metabolic shift from glycolysis to pentose phosphate pathway in the human bladder cancer cell line RT4. Scientific Reports, 7(1), 9773. https://dx.doi.org/10.1038/s41598-017-09936-1

MLA:
Verma, Nisha, et al. "Benzo[a]pyrene-induced metabolic shift from glycolysis to pentose phosphate pathway in the human bladder cancer cell line RT4." Scientific Reports 7.1 (2017): 9773.

BibTeX: 

Last updated on 2019-28-02 at 06:23