Gamma interferon-induced guanylate binding protein 1 is a novel actin cytoskeleton remodeling factor

Journal article


Publication Details

Author(s): Ostler N, Britzen-Laurent N, Liebl A, Naschberger E, Lochnit G, Ostler M, Forster F, Kunzelmann P, Ince S, Supper V, Praefcke GJK, Schubert DW, Stockinger H, Herrmann C, Stürzl M
Journal: Molecular and Cellular Biology
Publication year: 2014
Volume: 34
Journal issue: 2
Pages range: 196-209
ISSN: 0270-7306


Abstract


Gamma interferon (IFN-?) regulates immune defenses against viruses, intracellular pathogens, and tumors by modulating cell proliferation, migration, invasion, and vesicle trafficking processes. The large GTPase guanylate binding protein 1 (GBP-1) is among the cellular proteins that is the most abundantly induced by IFN-? and mediates its cell biologic effects. As yet, the molecular mechanisms of action of GBP-1 remain unknown. Applying an interaction proteomics approach, we identified actin as a strong and specific binding partner of GBP-1. Furthermore, GBP-1 colocalized with actin at the subcellular level and was both necessary and sufficient for the extensive remodeling of the fibrous actin structure observed in IFN-?-exposed cells. These effects were dependent on the oligomerization and the GTPase activity of GBP-1. Purified GBP-1 and actin bound to each other, and this interaction was sufficient to impair the formation of actin filaments in vitro, as demonstrated by atomic force microscopy, dynamic light scattering, and fluorescence-monitored polymerization. Cosedimentation and band shift analyses demonstrated that GBP-1 binds robustly to globular actin and slightly to filamentous actin. This indicated that GBP-1 may induce actin remodeling via globular actin sequestering and/or filament capping. These results establish GBP-1 as a novel member within the family of actin-remodeling proteins specifically mediating IFN-?-dependent defense strategies.



FAU Authors / FAU Editors

Kunzelmann, Peter
Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe)
Liebl, Andrea
Professur für Molekulare und Experimentelle Chirurgie
Naschberger, Elisabeth PD Dr.
Medizinische Fakultät
Ostler, Nicole
Professur für Molekulare und Experimentelle Chirurgie
Schubert, Dirk W. Prof. Dr.
Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe)
Stürzl, Michael Prof. Dr.
Professur für Molekulare und Experimentelle Chirurgie


External institutions with authors

Justus-Liebig-Universität Gießen
Medizinische Universität Wien
Ruhr-Universität Bochum (RUB)
Technische Universität Chemnitz
Universität Köln


How to cite

APA:
Ostler, N., Britzen-Laurent, N., Liebl, A., Naschberger, E., Lochnit, G., Ostler, M.,... Stürzl, M. (2014). Gamma interferon-induced guanylate binding protein 1 is a novel actin cytoskeleton remodeling factor. Molecular and Cellular Biology, 34(2), 196-209. https://dx.doi.org/10.1128/MCB.00664-13

MLA:
Ostler, Nicole, et al. "Gamma interferon-induced guanylate binding protein 1 is a novel actin cytoskeleton remodeling factor." Molecular and Cellular Biology 34.2 (2014): 196-209.

BibTeX: 

Last updated on 2018-08-10 at 08:30