Garcia-Cuellar MP, Zilles O, Schreiner S, Birke M, Winkler T, Slany R (2001)
Publication Type: Journal article
Publication year: 2001
Publisher: Nature Publishing Group: Open Access Hybrid Model Option B
Book Volume: 20
Pages Range: 411-419
The translocation t(11;19) is frequently found in acute leukemia in infants. This event truncates the protooncogene MLL and fuses the 5′ end of MLL in frame with the ENL gene. ENL contributes a crucial protein-protein interaction domain to the resulting oncoprotein MLL-ENL. Here we show by yeast two-hybrid assays, GST-pull-down experiments and in a far western blot analysis that this domain is necessary and sufficient to recruit a novel member of the human Polycomb protein family (hPc3). hPc3 RNA was detected throughout the human hematopoietic system. Similar to other Polycomb proteins hPc3 acts as a transcriptional repressor. The ENL-hPc3 interaction was verified by mutual coprecipitation of the proteins from cell extracts. ENL and hPc3 tagged with fluorescent proteins co-localized in living cells in a nuclear dot pattern. An internal region of hPc3 was responsible for binding to ENL. Finally, hPc3 binds to the C-terminus of AF9, another common MLL fusion partner. The recruitment of a repressive function by ENL opens up a new insight into a possible mechanism of leukemogenesis by the fusion protein MLL-ENL.
APA:
Garcia-Cuellar, M.-P., Zilles, O., Schreiner, S., Birke, M., Winkler, T., & Slany, R. (2001). The ENL moiety of the childhood leukemia-associated MLL-ENL oncoprotein recruits human Polycomb 3. Oncogene, 20, 411-419. https://doi.org/10.1038/sj.onc.1204108
MLA:
Garcia-Cuellar, Maria-Paz, et al. "The ENL moiety of the childhood leukemia-associated MLL-ENL oncoprotein recruits human Polycomb 3." Oncogene 20 (2001): 411-419.
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