Home Publications Research Grants Inventions & Patents Awards Additional Research Activities Faculties & Institutions Research Areas

Evaluation of two different analytical methods for circulating tumor cell detection in peripheral blood of patients with primary breast cancer

Jaeger BAS, Jueckstock J, Andergassen U, Salmen J, Schochter F, Fink V, Alunni-Fabbroni M, Rezai M, Beck T, Beckmann M, Friese K, Friedl TWP, Janni W, Rack B (2014)

---

Publication Type: Journal article

Publication year: 2014

Journal

Book Volume: 2014

Pages Range: 491459

DOI: 10.1155/2014/491459

Abstract

Evidence is accumulating that circulating tumor cells (CTC) out of peripheral blood can serve as prognostic marker not only in metastatic but also in early breast cancer (BC). Various methods are available to detect CTC. Comparisons between the different techniques, however, are rare.We evaluate two different methods for CTC enrichment and detection in primary BC patients: the FDA-approved CellSearch System (CSS; Veridex, Warren, USA) and a manual immunocytochemistry (MICC). The cut-off value for positivity was >=1 CTC.The two different nonoverlapping patient cohorts evaluated with one or the other method were well balanced regarding common clinical parameters. Before adjuvant CHT 21.1% (416 out of 1972) and 20.6% (247 out of 1198) of the patients were CTC-positive, while after CHT 22.5% (359 out of 1598) and 16.6% (177 out of 1066) of the patients were CTC-positive using CSS or MICC, respectively. CTC positivity rate before CHT was thus similar and not significantly different (P = 0.749), while CTC positivity rate immediately after CHT was significantly lower using MICC compared to CSS (P < 0.001).Using CSS or MICC for CTC detection, we found comparable prevalence of CTC before but not after adjuvant CHT.

Authors with CRIS profile

Involved external institutions

How to cite

APA:

Jaeger, B.A.S., Jueckstock, J., Andergassen, U., Salmen, J., Schochter, F., Fink, V.,... Rack, B. (2014). Evaluation of two different analytical methods for circulating tumor cell detection in peripheral blood of patients with primary breast cancer. BioMed Research International, 2014, 491459. https://doi.org/10.1155/2014/491459

MLA:

Jaeger, B. A. S., et al. "Evaluation of two different analytical methods for circulating tumor cell detection in peripheral blood of patients with primary breast cancer." BioMed Research International 2014 (2014): 491459.

BibTeX: Download