Stein MF, Blume K, Heilingloh C, Kummer M, Biesinger B, Sticht H, Steinkasserer A (2015)
Publication Type: Journal article
Publication year: 2015
Book Volume: 459
Pages Range: 42-8
Journal Issue: 1
DOI: 10.1016/j.bbrc.2015.02.057
CD83 is one of the best known surface markers for mature human dendritic cells (DCs). The full-length 45 kDa type-I membrane-bound form (mbCD83) is strongly glycosylated upon DCs maturation. As co-stimulatory properties of CD83 are attributed to mbCD83 surface expression is required for efficient T-cell stimulation by mature DCs. By yeast two-hybrid screening, we were able to identify GRASP55 as interaction partner of CD83. DCs maturation induces endogenous CD83 protein expression with simultaneous regulation of CD83 glycosylation, interaction and co-localization with GRASP55 and CD83 surface exposure. GRASP55 is especially known for its role in maintaining Golgi architecture, but also plays a role in Golgi transport of specific cargo proteins bearing a C-terminal valine residue. Here we additionally demonstrate that binding of CD83 and GRASP55 rely on the C-terminal TELV-motif of CD83. Mutation of this TELV-motif not only disrupted binding to GRASP55, but also altered the glycosylation pattern of CD83 and reduced its membrane expression. Here we show for the first time that GRASP55 interacts with CD83 shortly after induction of DC maturation and that this interaction plays a role in CD83 glycosylation as well as in surface expression of CD83 on DCs.
APA:
Stein, M.F., Blume, K., Heilingloh, C., Kummer, M., Biesinger, B., Sticht, H., & Steinkasserer, A. (2015). CD83 and GRASP55 interact in human dendritic cells. Biochemical and Biophysical Research Communications, 459(1), 42-8. https://dx.doi.org/10.1016/j.bbrc.2015.02.057
MLA:
Stein, Marcello F., et al. "CD83 and GRASP55 interact in human dendritic cells." Biochemical and Biophysical Research Communications 459.1 (2015): 42-8.
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