Adhesion/growth-regulatory galectins in the human eye: localization profiles and tissue reactivities as a standard to detect disease-associated alterations

Schlötzer-Schrehardt U, Andre S, Janko C, Kaltner H, Kopitz J, Gabius HJ, Herrmann M (2012)

Publication Type: Journal article

Publication year: 2012

Journal

Graefes Archive For Clinical and Experimental Ophthalmology Springer Verlag (Germany)

Book Volume: 250

Pages Range: 1169-80

Journal Issue: 8

DOI: 10.1007/s00417-012-2021-9

Abstract

To characterize the localization profiles of seven adhesion/growth-regulatory galectins in the normal human eye using immunodetection of endogenous galectins and binding of labeled galectins to sections, useful as a basis to detect disease-associated alterations.Non-cross-reactive anti-galectin antibodies and biotinylated galectins were tested on acetone-fixed cryosections of normal human donor eyes. Controls included omission of first-step reagent, testing of an antibody against a galectin specific for rat (galectin-5), and blocking of galectin binding with lactose.Galectin presence was not restricted to one or few members of this family. Signal occurrence can even include all tested or most proteins (conjunctival or corneal epithelium), whereas choroid positivity is fully accounted for by galectin-9. Regional specificity and characteristic profiles for each protein, immuno- and galectin histochemically, were determined. Differences in tissue reactivity among the galectins were detected.That the galectins have characteristic localization/reactivity profiles supports the concept of a network with potential for non-overlapping functions. The reported data thus prompt to proceed to the respective analysis of specimens from ocular diseases.

Authors with CRIS profile

Ursula Schlötzer-Schrehardt Medizinische Fakultät Christina Janko Department of Otorhinolaryngology – Head and Neck Surgery Martin Herrmann Department of Medicine 3 – Rheumatology and Immunology

Additional Organisation(s)

Department of Ophthalmology

Involved external institutions

Ludwig-Maximilians-Universität (LMU) DE Germany (DE) Ruprecht-Karls-Universität Heidelberg DE Germany (DE)

How to cite

APA:

Schlötzer-Schrehardt, U., Andre, S., Janko, C., Kaltner, H., Kopitz, J., Gabius, H.-J., & Herrmann, M. (2012). Adhesion/growth-regulatory galectins in the human eye: localization profiles and tissue reactivities as a standard to detect disease-associated alterations. Graefes Archive For Clinical and Experimental Ophthalmology, 250(8), 1169-80. https://dx.doi.org/10.1007/s00417-012-2021-9

MLA:

Schlötzer-Schrehardt, Ursula, et al. "Adhesion/growth-regulatory galectins in the human eye: localization profiles and tissue reactivities as a standard to detect disease-associated alterations." Graefes Archive For Clinical and Experimental Ophthalmology 250.8 (2012): 1169-80.

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