Tangential Flow Ultrafiltration Allows Purification and Concentration of Lauric Acid-/Albumin-Coated Particles for Improved Magnetic Treatment

Zaloga J, Stapf M, Nowak J, Poettler M, Friedrich RP, Tietze R, Lyer S, Lee G, Odenbach S, Hilger I, Alexiou C (2015)

Publication Type: Journal article

Publication year: 2015

Journal

International Journal of Molecular Sciences MDPI

Book Volume: 16

Pages Range: 19291-307

Journal Issue: 8

DOI: 10.3390/ijms160819291

Abstract

Superparamagnetic iron oxide nanoparticles (SPIONs) are frequently used for drug targeting, hyperthermia and other biomedical purposes. Recently, we have reported the synthesis of lauric acid-/albumin-coated iron oxide nanoparticles SEON(LA-BSA), which were synthesized using excess albumin. For optimization of magnetic treatment applications, SPION suspensions need to be purified of excess surfactant and concentrated. Conventional methods for the purification and concentration of such ferrofluids often involve high shear stress and low purification rates for macromolecules, like albumin. In this work, removal of albumin by low shear stress tangential ultrafiltration and its influence on SEON(LA-BSA) particles was studied. Hydrodynamic size, surface properties and, consequently, colloidal stability of the nanoparticles remained unchanged by filtration or concentration up to four-fold (v/v). Thereby, the saturation magnetization of the suspension can be increased from 446.5 A/m up to 1667.9 A/m. In vitro analysis revealed that cellular uptake of SEON(LA-BSA) changed only marginally. The specific absorption rate (SAR) was not greatly affected by concentration. In contrast, the maximum temperature Tmax in magnetic hyperthermia is greatly enhanced from 44.4 °C up to 64.9 °C by the concentration of the particles up to 16.9 mg/mL total iron. Taken together, tangential ultrafiltration is feasible for purifying and concentrating complex hybrid coated SPION suspensions without negatively influencing specific particle characteristics. This enhances their potential for magnetic treatment.

Authors with CRIS profile

Jan Zaloga Department of Otorhinolaryngology – Head and Neck Surgery Rainer Tietze Department of Otorhinolaryngology – Head and Neck Surgery Stefan Lyer Department of Otorhinolaryngology – Head and Neck Surgery Geoffrey Lee Lehrstuhl für Pharmazeutische Technologie und Biopharmazie Christoph Alexiou Professur für Nanomedizin

Involved external institutions

Universitätsklinikum Jena DE Germany (DE) Technische Universität Dresden DE Germany (DE)

How to cite

APA:

Zaloga, J., Stapf, M., Nowak, J., Poettler, M., Friedrich, R.P., Tietze, R.,... Alexiou, C. (2015). Tangential Flow Ultrafiltration Allows Purification and Concentration of Lauric Acid-/Albumin-Coated Particles for Improved Magnetic Treatment. International Journal of Molecular Sciences, 16(8), 19291-307. https://doi.org/10.3390/ijms160819291

MLA:

Zaloga, Jan, et al. "Tangential Flow Ultrafiltration Allows Purification and Concentration of Lauric Acid-/Albumin-Coated Particles for Improved Magnetic Treatment." International Journal of Molecular Sciences 16.8 (2015): 19291-307.

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