Villin-like actin-binding proteins are expressed ubiquitously in Arabidopsis

Journal article

Publication Details

Author(s): Klahre U, Friederich E, Kost B, Louvard D, Chua NH
Journal: Plant Physiology
Publisher: American Society of Plant Biologists
Publication year: 2000
Volume: 122
Journal issue: 1
Pages range: 35-47
ISSN: 0032-0889


In an attempt to elucidate the biological function of villin-like actin-binding proteins in plants we have cloned several genes encoding Arabidopsis proteins with high homology to animal villin. We found that Arabidopsis contains at least four villin-like genes (AtVLNs) encoding four different VLN isoforms. Two AtVLN isoforms are more closely related to mammalian villin in their primary structure and are also antigenically related, whereas the other two contain significant changes in the C-terminal headpiece domain. RNA and promoter/beta-glucuronidase expression studies demonstrated that AtVLN genes are expressed in all organs, with elevated expression levels in certain types of cells. These results suggest that AtVLNs have less-specialized functions than mammalian villin, which is found only in the microvilli of brush border cells, Immunoblot experiments using a monoclonal antibody against pig villin showed that AtVLNs are widely distributed in a variety of plant tissues. Green fluorescent protein fused to full-length AtVLN and individual AtVLN headpiece domains can bind to both animal and plant actin filaments in vivo.

External institutions with authors

Institut Curie
Rockefeller University

How to cite

Klahre, U., Friederich, E., Kost, B., Louvard, D., & Chua, N.-H. (2000). Villin-like actin-binding proteins are expressed ubiquitously in Arabidopsis. Plant Physiology, 122(1), 35-47.

Klahre, Ulrich, et al. "Villin-like actin-binding proteins are expressed ubiquitously in Arabidopsis." Plant Physiology 122.1 (2000): 35-47.


Last updated on 2018-10-08 at 10:42