Nanoparticles size-dependently initiate self-limiting NETosis-driven inflammation

Munoz LE, Bilyy R, Biermann MHC, Kienhoefer D, Maueröder C, Hahn J, Brauner JM, Weidner D, Chen J, Scharin-Mehlmann M, Janko C, Friedrich RP, Mielenz D, Dumych T, Lootsik MD, Schauer C, Schett G, Hoffmann M, Zhao Y, Herrmann M (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Proceedings of the National Academy of Sciences of the United States of America National Academy of Sciences

Book Volume: 113

Pages Range: E5856-E5865

Journal Issue: 40

DOI: 10.1073/pnas.1602230113

Abstract

The critical size for strong interaction of hydrophobic particles with phospholipid bilayers has been predicted to be 10 nm. Because of the wide spreading of nonpolar nanoparticles (NPs) in the environment, we aimed to reveal the ability of living organisms to entrap NPs via formation of neutrophil extracellular traps (NETs). Upon interaction with various cell types and tissues, 10- to 40-nm-sized NPs induce fast (<20 min) damage of plasma membranes and instability of the lysosomal compartment, leading to the immediate formation of NETs. In contrast, particles sized 100-1,000 nm behaved rather inertly. Resulting NET formation (NETosis) was accompanied by an inflammatory reaction intrinsically endowed with its own resolution, demonstrated in lungs and air pouches of mice. Persistence of small NPs in joints caused unremitting arthritis and bone remodeling. Small NPs coinjected with antigen exerted adjuvant-like activity. This report demonstrates a cellular mechanism that explains how small NPs activate the NETosis pathway and drive their entrapping and resolution of the initial inflammatory response.

Authors with CRIS profile

Christian Maueröder Department of Medicine 3 – Rheumatology and Immunology Jonas Hahn Department of Medicine 3 – Rheumatology and Immunology Juan Chen Lehrstuhl für Neurochirurgie Marina Scharin-Mehlmann Lehrstuhl für Elektronische Bauelemente Dirk Mielenz Medizinische Fakultät Georg Schett Lehrstuhl für Innere Medizin III Markus Hoffmann Department of Medicine 3 – Rheumatology and Immunology Martin Herrmann Department of Medicine 3 – Rheumatology and Immunology

Additional Organisation(s)

Exzellenz-Cluster Engineering of Advanced Materials

Involved external institutions

Danylo Halytsky Lviv National Medical University / Львiвський Національний Медичний Унiверситет iм. Данила Галицького UA Ukraine (UA)

How to cite

APA:

Munoz, L.E., Bilyy, R., Biermann, M.H.C., Kienhoefer, D., Maueröder, C., Hahn, J.,... Herrmann, M. (2016). Nanoparticles size-dependently initiate self-limiting NETosis-driven inflammation. Proceedings of the National Academy of Sciences of the United States of America, 113(40), E5856-E5865. https://doi.org/10.1073/pnas.1602230113

MLA:

Munoz, Luis E., et al. "Nanoparticles size-dependently initiate self-limiting NETosis-driven inflammation." Proceedings of the National Academy of Sciences of the United States of America 113.40 (2016): E5856-E5865.

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