Molecular Dynamics Characterization of the Structures and Induction Mechanisms of a Reverse Phenotype of the Tetracycline Receptor

Journal article


Publication Details

Author(s): Seidel U, Othersen O, Lanig H, Beierlein F, Clark T
Journal: Journal of Physical Chemistry B
Publisher: American Chemical Society
Publication year: 2007
Volume: 111
Journal issue: 21
Pages range: 6006-6014
ISSN: 1520-6106
eISSN: 1520-5207


Abstract


Molecular-dynamics simulations have been used to investigate the mechanism of induction of a mutant (revTetR) of the tetracycline repressor protein (TetR) that shows the reverse phenotype (i.e., it is induced in the absence of tetracyclines and not in their presence). Low-frequency, normal-mode analyses demonstrate that the reverse phenotype is reproduced by the simulations on the basis of criteria established for wild-type TetR. The reverse phenotype is caused by the fact that the DNA-binding heads in revTetR are closer than the ideal distance needed for DNA-binding when no inducer is present. This distance increases on binding an inducer. Whereas this distance increase makes the interhead distance too large in wild-type TetR, it increases to the ideal value in revTetR. Thus, the mechanism of induction is the same for the two proteins, but the consequences are reversed because of the smaller interhead distance in revTetR when no inducer is present.



FAU Authors / FAU Editors

Beierlein, Frank Dr.
Computer-Chemie-Centrum
Clark, Timothy apl. Prof. Dr.
Computer-Chemie-Centrum
Lanig, Harald PD Dr.
Computer-Chemie-Centrum


How to cite

APA:
Seidel, U., Othersen, O., Lanig, H., Beierlein, F., & Clark, T. (2007). Molecular Dynamics Characterization of the Structures and Induction Mechanisms of a Reverse Phenotype of the Tetracycline Receptor. Journal of Physical Chemistry B, 111(21), 6006-6014. https://dx.doi.org/10.1021/jp0674468

MLA:
Seidel, Ute, et al. "Molecular Dynamics Characterization of the Structures and Induction Mechanisms of a Reverse Phenotype of the Tetracycline Receptor." Journal of Physical Chemistry B 111.21 (2007): 6006-6014.

BibTeX: 

Last updated on 2018-10-08 at 05:55