Novel technique for high-precision stereotactic irradiation of mouse brains

Hartmann J, Wölfelschneider J, Stache C, Buslei R, Derer A, Schwarz M, Bäuerle T, Fietkau R, Gaipl U, Bert C, Hoelsken A, Frey B (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Strahlentherapie und Onkologie Urban & Vogel

Book Volume: 192

Pages Range: 806-814

Journal Issue: 11

DOI: 10.1007/s00066-016-1014-8

Abstract

Small animal irradiation systems were developed for preclinical evaluation of tumor therapy closely resembling the clinical situation. Mostly only clinical LINACs are available, so protocols for small animal partial body irradiation using a conventional clinical system are essential. This study defines a protocol for conformal brain tumor irradiations in mice.CT and MRI images were used to demarcate the target volume and organs at risk. Three 6 MV photon beams were planned for a total dose of 10 fractions of 1.8 Gy. The mouse position in a dedicated applicator was verified by an X-ray patient positioning system before each irradiation. Dosimetric verifications (using ionization chambers and films) were performed. Irradiation-induced DNA damage was analyzed to verify the treatment effects on the cellular level.The defined treatment protocol and the applied fractionation scheme were feasible. The in-house developed applicator was suitable for individual positioning at submillimeter accuracy of anesthetized mice during irradiation, altogether performed in less than 10 min. All mice tolerated the treatment well. Measured dose values perfectly matched the nominal values from treatment planning. Cellular response was restricted to the target volume.Clinical LINAC-based irradiations of mice offer the potential to treat orthotopic tumors conformably. Especially with respect to lateral penumbra, dedicated small animal irradiation systems exceed the clinical LINAC solution.

Authors with CRIS profile

Josefin Hartmann Department of Radiation Oncology Jens Wölfelschneider Department of Radiation Oncology Christina Stache Lehrstuhl für Neuropathologie Anja Derer Department of Radiation Oncology Martin Schwarz Professur für Experimentelle Augenheilkunde Tobias Bäuerle Professur für Multimodale Bildgebung in der präklinischen Forschung Rainer Fietkau Lehrstuhl für Strahlentherapie Udo Gaipl Department of Radiation Oncology Christoph Bert Professur für Med. Strahlenphysik Benjamin Frey Department of Radiation Oncology

How to cite

APA:

Hartmann, J., Wölfelschneider, J., Stache, C., Buslei, R., Derer, A., Schwarz, M.,... Frey, B. (2016). Novel technique for high-precision stereotactic irradiation of mouse brains. Strahlentherapie und Onkologie, 192(11), 806-814. https://doi.org/10.1007/s00066-016-1014-8

MLA:

Hartmann, Josefin, et al. "Novel technique for high-precision stereotactic irradiation of mouse brains." Strahlentherapie und Onkologie 192.11 (2016): 806-814.

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