Comparative whole genome analysis of three consecutive Salmonella diarizonae isolates

Gerlach RG, Walter S, McClelland M, Schmidt C, Steglich M, Prager R, Bender JK, Fuchs S, Schoerner C, Rabsch W, Lang W, Jantsch J (2017)

Publication Type: Journal article

Publication year: 2017

Journal

International Journal of Medical Microbiology Elsevier

DOI: 10.1016/j.ijmm.2017.09.001

Abstract

Infections of very young children or immunocompromised people with Salmonella of higher subspecies are a well-known phenomenon often associated with contact to cold-blooded animals. We describe the molecular characterization of three S. enterica subsp. diarizonae strains, isolated consecutively over a period of several months from a hospital patient suffering from diarrhea and sepsis with fatal outcome. With the initial isolate the first complete genome sequence of a member of subsp. diarizonae is provided and based on this reference we revealed the genomic differences between the three isolates by use of next-generation sequencing and confirmed by phenotypical tests. Genome comparisons revealed mutations within gpt, hfq and purK in the first isolate as a sign of clonal variation rather than host-directed evolution. Furthermore, our work demonstrates that S. enterica subsp. diarizonae possess, besides a conserved set of known Salmonella Pathogenicity Islands, a variable portfolio of additional genomic islands of unknown function.

Authors with CRIS profile

Roman Gerlach Institute of Microbiology – Clinical Microbiology, Immunology and Hygiene Werner Lang Department of Vascular Surgery

Involved external institutions

Robert Koch-Institut (RKI) DE Germany (DE) Universität Regensburg DE Germany (DE) University of California Irvine US United States (USA) (US)

How to cite

APA:

Gerlach, R.G., Walter, S., McClelland, M., Schmidt, C., Steglich, M., Prager, R.,... Jantsch, J. (2017). Comparative whole genome analysis of three consecutive Salmonella diarizonae isolates. International Journal of Medical Microbiology. https://dx.doi.org/10.1016/j.ijmm.2017.09.001

MLA:

Gerlach, Roman G., et al. "Comparative whole genome analysis of three consecutive Salmonella diarizonae isolates." International Journal of Medical Microbiology (2017).

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