Characterization of the Protein Tyrosine Phosphatase LmPRL-1 Secreted by Leishmania major via the Exosome Pathway

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autorinnen und Autoren: Leitherer S, Clos J, Liebler-Tenorio EM, Schleicher U, Bogdan C, Soulat D
Zeitschrift: Infection and Immunity
Jahr der Veröffentlichung: 2017
Band: 85
Heftnummer: 8
ISSN: 0019-9567


Abstract


Similar to other intracellular pathogens, Leishmania parasites are known to evade the antimicrobial effector functions of host immune cells. To date, however, only a few virulence factors have been described for Leishmania major, one of the causative agents of cutaneous leishmaniasis. Here, we have characterized the expression and function of an L. major phosphatase, which we termed LmPRL-1. This enzyme shows a strong structural similarity to the human phosphatases of regenerating liver (PRL-1, -2, and -3) that regulate the proliferation, differentiation, and motility of cells. The biochemical characterization of the L. major phosphatase revealed that the enzyme is redox sensitive. When analyzing the subcellular localization of LmPRL-1 in promastigotes, amastigotes, and infected macrophages, we found that the phosphatase was predominantly expressed and secreted by promastigotes via the exosome route. Finally, we observed that ectopic expression of LmPRL-1 in L. major led to an increased number of parasites in macrophages. From these data, we conclude that the L. major phosphatase LmPRL-1 contributes to the intracellular survival of the parasites in macrophages.



FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Bogdan, Christian Prof. Dr.
Lehrstuhl für Mikrobiologie und Infektionsimmunologie
Leitherer, Sabine
Lehrstuhl für Mikrobiologie und Infektionsimmunologie
Schleicher, Ulrike PD Dr.
Mikrobiologisches Institut - Klinische Mikrobiologie, Immunologie und Hygiene


Einrichtungen weiterer Autorinnen und Autoren

Bernhard-Nocht-Institut für Tropenmedizin (BNITM) / Bernhard Nocht Institute for Tropical Medicine
Friedrich-Loeffler-Institut (FLI), Bundesforschungsinstitut für Tiergesundheit


Zitierweisen

APA:
Leitherer, S., Clos, J., Liebler-Tenorio, E.M., Schleicher, U., Bogdan, C., & Soulat, D. (2017). Characterization of the Protein Tyrosine Phosphatase LmPRL-1 Secreted by Leishmania major via the Exosome Pathway. Infection and Immunity, 85(8). https://dx.doi.org/10.1128/IAI.00084-17

MLA:
Leitherer, Sabine, et al. "Characterization of the Protein Tyrosine Phosphatase LmPRL-1 Secreted by Leishmania major via the Exosome Pathway." Infection and Immunity 85.8 (2017).

BibTeX: 

Zuletzt aktualisiert 2018-08-10 um 08:23