Functional Evaluation of Activation-dependent Alterations in the Sialoglycan Composition of T Cells

Naito-Matsui Y, Takada S, Kano Y, Iyoda T, Sugai M, Shimizu A, Inaba K, Nitschke L, Tsubata T, Oka S, Kozutsumi Y, Takematsu H (2014)

Publication Status: Published

Publication Type: Journal article

Publication year: 2014

Journal

Journal of Biological Chemistry American Society for Biochemistry and Molecular Biology

Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Book Volume: 289

Pages Range: 1564-1579

Journal Issue: 3

DOI: 10.1074/jbc.M113.523753

Abstract

Sialic acids (Sias) are often conjugated to the termini of cellular glycans and are key mediators of cellular recognition. Sias are nine-carbon acidic sugars, and, in vertebrates, the major species are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), differing in structure at the C5 position. Previously, we described a positive feedback loop involving regulation of Neu5Gc expression in mouse B cells. In this context, Neu5Gc negatively regulated B-cell proliferation, and Neu5Gc expression was suppressed upon activation. Similarly, resting mouse T cells expressed principally Neu5Gc, and Neu5Ac was induced upon activation. In the present work, we used various probes to examine sialoglycan expression by activated T cells in terms of the Sia species expressed and the linkages of Sias to glycans. Upon T-cell activation, sialoglycan expression shifted from Neu5Gc to Neu5Ac, and the linkage shifted from 2,6 to 2,3. These changes altered the expression levels of sialic acid-binding immunoglobulin-like lectin (siglec) ligands. Expression of sialoadhesin and Siglec-F ligands increased, and that of CD22 ligands decreased. Neu5Gc exerted a negative effect on T-cell activation, both in terms of the proliferative response and in the context of activation marker expression. Suppression of Neu5Gc expression in mouse T and B cells prevented the development of nonspecific CD22-mediated T cell-B cell interactions. Our results suggest that an activation-dependent shift from Neu5Gc to Neu5Ac and replacement of 2,6 by 2,3 linkages may regulate immune cell interactions at several levels.

Authors with CRIS profile

Lars Nitschke Professur für Genetik

Involved external institutions

Kyoto University / 京都大学 Kyōto daigaku JP Japan (JP) Kyoto University Hospital / 京都大学医学部附属病院 JP Japan (JP) Tokyo Medical and Dental University (TMDU) / 東京医科歯科大学 JP Japan (JP)

How to cite

APA:

Naito-Matsui, Y., Takada, S., Kano, Y., Iyoda, T., Sugai, M., Shimizu, A.,... Takematsu, H. (2014). Functional Evaluation of Activation-dependent Alterations in the Sialoglycan Composition of T Cells. Journal of Biological Chemistry, 289(3), 1564-1579. https://dx.doi.org/10.1074/jbc.M113.523753

MLA:

Naito-Matsui, Yuko, et al. "Functional Evaluation of Activation-dependent Alterations in the Sialoglycan Composition of T Cells." Journal of Biological Chemistry 289.3 (2014): 1564-1579.

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