Measuring the Invisible: Determining the Size of Growing Nanodomains Using the "Inverse FCS"

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Details zur Publikation

Autorinnen und Autoren: Fenz SF, Smith AS, Monzel C
Zeitschrift: Biophysical Journal
Verlag: CELL PRESS
Jahr der Veröffentlichung: 2017
Band: 112
Heftnummer: 11
Seitenbereich: 2245-2246
ISSN: 0006-3495
Sprache: Englisch


Abstract


Molecular recruitment into functional domains in the cell plasma membrane is an important prerequisite for many cellular processes, such as cell signaling, cell-matrix adhesion, or the formation of synapses. High precision measurements of the origin and evolution of these structures is key to their understanding; however, experimental methods with which one can detect molecular dynamics and aggregation, with microsecond temporal resolution and at nanometer scales, are scarce.One of the most developed approaches, which is up to the task, is fluorescence correlation spectroscopy (FCS). In FCS, fluorescent particles diffuse through a laser excitation beam and emit pulses of light proportional to the particle brightness.One way to circumvent the issue of relating probe intensities to their number is to use inverse fluorescence correlation spectroscopy (iFCS). Here, as opposed to conventional FCS, the molecule of interest is not labeled but diffuses in a homogeneously fluorescent environment, giving rise to negative stochastic intensity fluctuations.



FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Smith, Ana-Suncana Prof. Dr.
Professur für Theoretische Physik


Zusätzliche Organisationseinheit(en)
Exzellenz-Cluster Engineering of Advanced Materials


Einrichtungen weiterer Autorinnen und Autoren

Julius-Maximilians-Universität Würzburg
University of Paris 6 - Pierre et Marie Curie / Université Paris VI Pierre et Marie Curie (UPMC)


Forschungsbereiche

A3 Multiscale Modeling and Simulation
Exzellenz-Cluster Engineering of Advanced Materials


Zitierweisen

APA:
Fenz, S.F., Smith, A.-S., & Monzel, C. (2017). Measuring the Invisible: Determining the Size of Growing Nanodomains Using the "Inverse FCS". Biophysical Journal, 112(11), 2245-2246. https://dx.doi.org/10.1016/j.bpj.2017.04.014

MLA:
Fenz, Susanne F., Ana-Suncana Smith, and Cornelia Monzel. "Measuring the Invisible: Determining the Size of Growing Nanodomains Using the "Inverse FCS"." Biophysical Journal 112.11 (2017): 2245-2246.

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Zuletzt aktualisiert 2018-10-08 um 21:41