Deletion of interleukin-6 signal transducer gp130 in small sensory neurons attenuates mechanonociception and down-regulates TRPA1 expression
Malsch P, Andratsch M, Vogl C, Link A, Alzheimer C, Brierley SM, Hughes PA, Kress M (2014)
Publication Type: Journal article
Publication year: 2014
Journal
Publisher: Society for Neuroscience
Book Volume: 34
Pages Range: 9845-56
Journal Issue: 30
DOI: 10.1523/JNEUROSCI.5161-13.2014
Abstract
Glycoprotein 130 (gp130) is the signal transducing receptor subunit for cytokines of the interleukin-6 (IL-6) family, and it is expressed in a multitude of cell types of the immune and nervous system. IL-6-like cytokines are not only key regulators of innate immunity and inflammation but are also essential factors for the differentiation and development of the somatosensory system. Mice with a null mutation of gp130 in primary nociceptive afferents (SNS-gp130(-/-)) are largely protected from hypersensitivity to mechanical stimuli in mouse models of pathological pain. Therefore, we set out to investigate how neuronal gp130 regulates mechanonociception. SNS-gp130(-/-) mice revealed reduced mechanosensitivity to high mechanical forces in the von Frey assay in vivo, and this was associated with a reduced sensitivity of nociceptive primary afferents in vitro. Together with these findings, transient receptor potential ankyrin 1 (TRPA1) mRNA expression was significantly reduced in DRG from SNS-gp130(-/-) mice. This was also reflected by a reduced number of neurons responding with calcium transients to TRPA1 agonists in primary DRG cultures. Downregulation of Trpa1 expression was predominantly discovered in nonpeptidergic neurons, with the deficit becoming evident during stages of early postnatal development. Regulation of Trpa1 mRNA expression levels downstream of gp130 involved the classical Janus kinase family-signal transducer and activator of transcription pathway. Our results closely link proinflammatory cytokines to the expression of TRPA1, both of which have been shown to contribute to hypersensitive pain states. We suggest that gp130 has an essential role in mechanonociception and in the regulation of TRPA1 expression.
Authors with CRIS profile
Andrea Link
Professur für Molekulare Medizin mit dem Schwerpunkt Molekulare Bildgebung
Christian Alzheimer
Lehrstuhl für Physiologie
Involved external institutions
Austria (AT)
Australia (AU)How to cite
APA:
Malsch, P., Andratsch, M., Vogl, C., Link, A., Alzheimer, C., Brierley, S.M.,... Kress, M. (2014). Deletion of interleukin-6 signal transducer gp130 in small sensory neurons attenuates mechanonociception and down-regulates TRPA1 expression. The Journal of Neuroscience, 34(30), 9845-56. https://dx.doi.org/10.1523/JNEUROSCI.5161-13.2014
MLA:
Malsch, Philipp, et al. "Deletion of interleukin-6 signal transducer gp130 in small sensory neurons attenuates mechanonociception and down-regulates TRPA1 expression." The Journal of Neuroscience 34.30 (2014): 9845-56.
BibTeX: Download