Cancer cell invasion and EMT marker expression: a three-dimensional study of the human cancer-host interface

Bronsert P, Enderle-Ammour K, Bader M, Timme S, Kuehs M, Csanadi A, Kayser G, Kohler I, Bausch D, Hoeppner J, Hopt UT, Keck T, Stickeler E, Passlick B, Schilling O, Reiss CP, Vashist Y, Brabletz T, Berger J, Lotz J, Olesch J, Werner M, Wellner UF (2014)

Publication Type: Journal article

Publication year: 2014

Journal

Journal of Pathology Wiley-Blackwell

Publisher: Wiley-Blackwell

Book Volume: 234

Pages Range: 410-22

Journal Issue: 3

DOI: 10.1002/path.4416

Abstract

Cancer cell invasion takes place at the cancer-host interface and is a prerequisite for distant metastasis. The relationships between current biological and clinical concepts such as cell migration modes, tumour budding and epithelial-mesenchymal transition (EMT) remains unclear in several aspects, especially for the 'real' situation in human cancer. We developed a novel method that provides exact three-dimensional (3D) information on both microscopic morphology and gene expression, over a virtually unlimited spatial range, by reconstruction from serial immunostained tissue slices. Quantitative 3D assessment of tumour budding at the cancer-host interface in human pancreatic, colorectal, lung and breast adenocarcinoma suggests collective cell migration as the mechanism of cancer cell invasion, while single cancer cell migration seems to be virtually absent. Budding tumour cells display a shift towards spindle-like as well as a rounded morphology. This is associated with decreased E-cadherin staining intensity and a shift from membranous to cytoplasmic staining, as well as increased nuclear ZEB1 expression.

Authors with CRIS profile

Thomas Brabletz Lehrstuhl für Experimentelle Medizin mit dem Schwerpunkt Molekulare Pathogeneseforschung

Involved external institutions

Fraunhofer-Institut für Bildgestützte Medizin (MEVIS) DE Germany (DE) Deutsches Krebsforschungszentrum (DKFZ) DE Germany (DE) Albert-Ludwigs-Universität Freiburg DE Germany (DE) Universitätsklinikum Hamburg-Eppendorf (UKE) DE Germany (DE)

How to cite

APA:

Bronsert, P., Enderle-Ammour, K., Bader, M., Timme, S., Kuehs, M., Csanadi, A.,... Wellner, U.F. (2014). Cancer cell invasion and EMT marker expression: a three-dimensional study of the human cancer-host interface. Journal of Pathology, 234(3), 410-22. https://doi.org/10.1002/path.4416

MLA:

Bronsert, P., et al. "Cancer cell invasion and EMT marker expression: a three-dimensional study of the human cancer-host interface." Journal of Pathology 234.3 (2014): 410-22.

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