Kit regulates HSC engraftment across the human-mouse species barrier

Cosgun KN, Rahmig S, Mende N, Reinke S, Hauber I, Schaefer C, Petzold A, Weisbach H, Heidkamp GF, Purbojo A, Cesnjevar R, Platz A, Bornhaeuser M, Schmitz M, Dudziak D, Hauber J, Kirberg J, Waskow C (2014)

Publication Type: Journal article

Publication year: 2014

Journal

Cell Stem Cell Elsevier (Cell Press)

Publisher: Elsevier (Cell Press)

Book Volume: 15

Pages Range: 227-38

Journal Issue: 2

DOI: 10.1016/j.stem.2014.06.001

Abstract

In-depth analysis of the cellular and molecular mechanisms regulating human HSC function will require a surrogate host that supports robust maintenance of transplanted human HSCs in vivo, but the currently available options are problematic. Previously we showed that mutations in the Kit receptor enhance engraftment of transplanted HSCs in the mouse. To generate an improved model for human HSC transplantation and analysis, we developed immune-deficient mouse strains containing Kit mutations. We found that mutation of the Kit receptor enables robust, uniform, sustained, and serially transplantable engraftment of human HSCs in adult mice without a requirement for irradiation conditioning. Using this model, we also showed that differential KIT expression identifies two functionally distinct subpopulations of human HSCs. Thus, we have found that the capacity of this Kit mutation to open up stem cell niches across species barriers has significant potential and broad applicability in human HSC research.

Authors with CRIS profile

Gordon Frederik Heidkamp Professur für Genetik Ariawan Purbojo Department of Paediatric Cardiac Surgery Robert Cesnjevar Professur für Herzchirurgie mit dem Schwerpunkt Kinderherzchirurgie Diana Dudziak Professur für die Biologie Dendritischer Zellen

Involved external institutions

Heinrich-Pette-Institut (HPI), Leibniz-Institut für Experimentelle Virologie / Heinrich Pette Institute – Leibniz Institute for Experimental Virology DE Germany (DE) Technische Universität Dresden DE Germany (DE) DKMS Deutsche Knochenmarkspenderdatei DE Germany (DE) Paul Ehrlich Institute / Paul-Ehrlich-Institut – Bundesinstitut für Impfstoffe und biomedizinische Arzneimittel (PEI) DE Germany (DE)

How to cite

APA:

Cosgun, K.N., Rahmig, S., Mende, N., Reinke, S., Hauber, I., Schaefer, C.,... Waskow, C. (2014). Kit regulates HSC engraftment across the human-mouse species barrier. Cell Stem Cell, 15(2), 227-38. https://doi.org/10.1016/j.stem.2014.06.001

MLA:

Cosgun, Kadriye Nehir, et al. "Kit regulates HSC engraftment across the human-mouse species barrier." Cell Stem Cell 15.2 (2014): 227-38.

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