Identification of the oleic acide ethanolamide (OEA) isomer cis-vaccenic acid ethanolamide (VEA) as a highly abundant 18:1 fatty acid ethanolamide in blood plasma from rats and humans

Journal article
(Original article)


Publication Details

Author(s): Röhrig W, Waibel R, Perlwitz C, Pischetsrieder M, Hoch T
Journal: Analytical and Bioanalytical Chemistry
Publisher: Springer Verlag (Germany)
Publication year: 2016
Volume: 408
Journal issue: 22
Pages range: 6141-6151
ISSN: 1618-2642
Language: English


Abstract


The endocannabinoid system is important in various physiological pathways, especially the regulation of food intake. It consists of endocannabinoids like 2-arachidonoyl-glycerol (2-AG) or the fatty acid ethanolamide archachidonoyl-ethanolamide (AEA) with binding affinity to cannabinoid receptors. Further, fatty acid ethanolamides (FAEAs) influence the endocannabinoid system without affecting cannabinoid receptors by using independent physiological pathways. Among FAEAs, oleic acid ethanolamide (OEA) gained importance because of its promising ability to reduce food intake. By ultrahigh-performance liquid chromatography–electrospray ionization–tandem mass spectrometry (UHPLC–ESI–MS/MS), we detected a chromatographically separated molecule in plasma samples from rats and humans with identical mass and fragmentation patterns as those of OEA. Via synthesis and extensive analysis of ethanolamides of different cis/trans- and position isomers of oleic acid (cis9-18:1), we could identify the unknown molecule as vaccenic acid (cis11-18:1) ethanolamide (VEA). In this study we identified VEA as the most abundant 18:1 FAEA in rat plasma and the second most abundant 18:1 FAEA in human plasma.



FAU Authors / FAU Editors

Hoch, Tobias Dr.
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)
Pischetsrieder, Monika Prof. Dr.
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)
Röhrig, Waldemar
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)
Waibel, Reiner Dr.
Lehrstuhl für Pharmazeutische Chemie


Additional Organisation
Emil-Fischer-Zentrum (Emil Fischer Center)


How to cite

APA:
Röhrig, W., Waibel, R., Perlwitz, C., Pischetsrieder, M., & Hoch, T. (2016). Identification of the oleic acide ethanolamide (OEA) isomer cis-vaccenic acid ethanolamide (VEA) as a highly abundant 18:1 fatty acid ethanolamide in blood plasma from rats and humans. Analytical and Bioanalytical Chemistry, 408(22), 6141-6151. https://dx.doi.org/10.1007/s00216-016-9720-8

MLA:
Röhrig, Waldemar, et al. "Identification of the oleic acide ethanolamide (OEA) isomer cis-vaccenic acid ethanolamide (VEA) as a highly abundant 18:1 fatty acid ethanolamide in blood plasma from rats and humans." Analytical and Bioanalytical Chemistry 408.22 (2016): 6141-6151.

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Last updated on 2018-26-06 at 13:23