Distribution of protein oxidation products in the proteome of thermally processed milk

Journal article


Publication Details

Author(s): Meyer B, Baum F, Vollmer G, Pischetsrieder M
Journal: Journal of Agricultural and Food Chemistry
Publisher: American Chemical Society
Publication year: 2012
Volume: 60
Journal issue: 29
Pages range: 7306-7311
ISSN: 0021-8561


Abstract


During thermal milk processing, severe oxidation can occur, which alters the technological and physiological properties of the milk proteins. Due to differences in composition and physicochemical properties, it can be expected that the particular milk proteins are differently affected by oxidative damage. Therefore, the protein-specific distribution of oxidation products in the heated milk proteome was investigated. Raw and heated milk samples were separated by one-dimensional gel electrophoresis. Protein oxidation was visualized by Western blot after derivatization of protein carbonyls with 2,4-dinitrophenylhydrazine. Thus, α-lactalbumin displayed enhanced oxidation compared to α-lactoglobulin, despite its lower concentration in milk. Highly selective oxidation was detected for a previously unassigned minor milk protein. The protein was identified by its peptide mass fingerprint as a variant of αS1-casein (αS1-casein*). Similar oxidation patterns were observed in several commercial milk products. © 2012 American Chemical Society.



FAU Authors / FAU Editors

Baum, Florian
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)
Pischetsrieder, Monika Prof. Dr.
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)
Vollmer, Gregor
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)


Additional Organisation
Emil-Fischer-Zentrum (Emil Fischer Center)


How to cite

APA:
Meyer, B., Baum, F., Vollmer, G., & Pischetsrieder, M. (2012). Distribution of protein oxidation products in the proteome of thermally processed milk. Journal of Agricultural and Food Chemistry, 60(29), 7306-7311. https://dx.doi.org/10.1021/jf301666r

MLA:
Meyer, Bianca, et al. "Distribution of protein oxidation products in the proteome of thermally processed milk." Journal of Agricultural and Food Chemistry 60.29 (2012): 7306-7311.

BibTeX: 

Last updated on 2018-19-04 at 02:43