Identification and quantification of the glucose degradation product glucosone in peritoneal dialysis fluids by HPLC/DAD/MSMS

Journal article


Publication Details

Author(s): Mittelmaier S, Fünfrocken M, Fenn D, Fichert T, Pischetsrieder M
Journal: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Publisher: Elsevier
Publication year: 2010
Volume: 878
Pages range: 877-882
ISSN: 1570-0232


Abstract


Glucose degradation products (GDPs) formed during heat sterilization of peritoneal dialysis (PD) fluids exert cytotoxic effects and promote the formation of advanced glycation end-products in the peritoneal cavity. As a result, long-term application of continuous ambulatory peritoneal dialysis is limited. The composition and concentration of GDPs in PD fluids must be known to evaluate their biological effects. The present study describes a targeted screening for novel GDPs in PD fluids. For this purpose, dicarbonyl compounds were converted with o-phenylenediamine to give the respective quinoxaline derivatives, which were selectively monitored by HPLC/diode array detector. Glucosone was thereby identified as a novel major GDP in PD fluids. Product identity was confirmed by LC/MSMS analysis using independently synthesized glucosone as a reference compound. Furthermore, a method was developed to quantify glucosone in PD fluids by HPLC/UV after derivatization with o-phenylenediamine. The method's limit of detection was 0.6 μM and the limit of quantitation 1.1 μM. A linear calibration curve was obtained between 1.1 and 113.9 μM (R2 = 0.9999). Analyzed at three different concentration levels, recovery varied between 95.6% and 102.0%. The coefficient of variation ranged between 0.4% and 4.7%. The method was then applied to the measurement of glucosone in typical PD fluids. Glucosone levels in double chamber bag PD fluids varied between not detectable and 6.7 μM. In single chamber bag fluids, glucosone levels ranged between 28.7 and 40.7 μM. © 2010 Elsevier B.V. All rights reserved.



FAU Authors / FAU Editors

Mittelmaier, Stefan
Graduiertenzentrum der FAU
Pischetsrieder, Monika Prof. Dr.
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)


Additional Organisation
Emil-Fischer-Zentrum (Emil Fischer Center)


How to cite

APA:
Mittelmaier, S., Fünfrocken, M., Fenn, D., Fichert, T., & Pischetsrieder, M. (2010). Identification and quantification of the glucose degradation product glucosone in peritoneal dialysis fluids by HPLC/DAD/MSMS. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 878, 877-882. https://dx.doi.org/10.1016/j.jchromb.2010.02.004

MLA:
Mittelmaier, Stefan, et al. "Identification and quantification of the glucose degradation product glucosone in peritoneal dialysis fluids by HPLC/DAD/MSMS." Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 878 (2010): 877-882.

BibTeX: 

Last updated on 2018-19-04 at 02:42