Compressed sensing electron tomography of needle-shaped biological specimens - Potential for improved reconstruction fidelity with reduced dose

Journal article


Publication Details

Author(s): Saghi Z, Divitini G, Winter B, Leary R, Spiecker E, Ducati C, Midgley PA
Journal: Ultramicroscopy
Publisher: Elsevier
Publication year: 2016
Volume: 160
Pages range: 230-238
ISSN: 0304-3991


Abstract


Electron tomography is an invaluable method for 3D cellular imaging. The technique is, however, limited by the specimen geometry, with a loss of resolution due to a restricted tilt range, an increase in specimen thickness with tilt, and a resultant need for subjective and time-consuming manual segmentation. Here we show that 3D reconstructions of needle-shaped biological samples exhibit isotropic resolution, facilitating improved automated segmentation and feature detection. By using scanning transmission electron tomography, with small probe convergence angles, high spatial resolution is maintained over large depths of field and across the tilt range. Moreover, the application of compressed sensing methods to the needle data demonstrates how high fidelity reconstructions may be achieved with far fewer images (and thus greatly reduced dose) than needed by conventional methods. These findings open the door to high fidelity electron tomography over critically relevant length-scales, filling an important gap between existing 3D cellular imaging techniques. (C) 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).



FAU Authors / FAU Editors

Spiecker, Erdmann Prof. Dr.
Lehrstuhl für Werkstoffwissenschaften (Mikro- und Nanostrukturforschung)
Apeleo Zubiri, Benjamin Dr.-Ing.
Lehrstuhl für Werkstoffwissenschaften (Mikro- und Nanostrukturforschung)


Additional Organisation
Exzellenz-Cluster Engineering of Advanced Materials
Interdisziplinäres Zentrum, Center for Nanoanalysis and Electron Microscopy (CENEM)
Lehrstuhl für Werkstoffwissenschaften (Mikro- und Nanostrukturforschung)


External institutions with authors

University of Cambridge


Research Fields

A2 Nanoanalysis and Microscopy
Exzellenz-Cluster Engineering of Advanced Materials


How to cite

APA:
Saghi, Z., Divitini, G., Winter, B., Leary, R., Spiecker, E., Ducati, C., & Midgley, P.A. (2016). Compressed sensing electron tomography of needle-shaped biological specimens - Potential for improved reconstruction fidelity with reduced dose. Ultramicroscopy, 160, 230-238. https://dx.doi.org/10.1016/j.ultramic.2015.10.021

MLA:
Saghi, Zineb, et al. "Compressed sensing electron tomography of needle-shaped biological specimens - Potential for improved reconstruction fidelity with reduced dose." Ultramicroscopy 160 (2016): 230-238.

BibTeX: 

Last updated on 2019-29-05 at 09:25