Effect of Digitalis lanata matrix composition on the lanatoside C partition coefficient and its consequence on rotation locular counter-current chromatography efficiency
Braga FC, Kreis W, Braga de Oliveira A (1998)
Publication Language: English
Publication Type: Journal article, Original article
Publication year: 1998
Journal
Publisher: Elsevier
Book Volume: 822
Pages Range: 37-44
DOI: 10.1016/S0021-9673(98)00559-7
Abstract
Lanatoside C standard samples had their partition coefficients (K) determined in dichloromethane-methanol-water (4:4:3), (5:6:4) and (7:13:8), by the shake flask method and the obtained values were similar. On the other hand, K for lanatoside C accomplished by calculation from rotation locular counter-current chromatography (RLCC) traces of Digitalis lanata pre-purified extracts, as well as by the shake flask method, in the same solvent systems, showed a significant variation. Such variation was attributed to differences in the pre-purification procedures for D. lanata extract, which resulted in starting materials for RLCC with very distinct cardenolide profiles, reflecting, thus, in the K values for lanatoside C and, consequently, in the lanatoside C purity at the end of the chromatographic process. Copyright (C) 1998 Elsevier Science B.V.
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APA:
Braga, F.C., Kreis, W., & Braga de Oliveira, A. (1998). Effect of Digitalis lanata matrix composition on the lanatoside C partition coefficient and its consequence on rotation locular counter-current chromatography efficiency. Journal of Chromatography A, 822, 37-44. https://dx.doi.org/10.1016/S0021-9673(98)00559-7
MLA:
Braga, Fernão C., Wolfgang Kreis, and Alaíde Braga de Oliveira. "Effect of Digitalis lanata matrix composition on the lanatoside C partition coefficient and its consequence on rotation locular counter-current chromatography efficiency." Journal of Chromatography A 822 (1998): 37-44.
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