Hydrodynamic impact of particle shape in slurry packed liquid chromatography columns
Lottes F, Arlt W, Minceva M, Stenby EH (2009)
Publication Status: Published
Publication Type: Journal article
Publication year: 2009
Journal
Publisher: Elsevier
Book Volume: 1216
Pages Range: 5687-5695
Journal Issue: 30
DOI: 10.1016/j.chroma.2009.05.090
Abstract
We report on a series of flow velocity and efficiency profiles, which were measured across the cross section of preparative chromatographic columns packed with different stationary phase materials using computed tomography. It is shown that this non-invasive technique is very useful for visualization of the inner part of a packed column and measurement of the spatial resolved column packing properties. For evaluation of the influence of the particle shape on the velocity distribution and column performance, irregular and spherical reversed phases were studied in detail. The results showed a decreasing velocity towards the column wall most certainly due to a lower permeability. This effect was much less pronounced in the case of spherical particles, indicating a more homogenous packing structure. The influence of the column packing pressure, as a possible measure for improvement of the packing homogeneity was also studied. It was shown that under the same packing conditions spherical particles always lead to a more homogeneous packing. The overall results of this work contribute to the origin of the fact that spherical material is superior to irregular one from the hydrodynamic point of view. (C) 2009 Elsevier B.V. All rights reserved.
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APA:
Lottes, F., Arlt, W., Minceva, M., & Stenby, E.H. (2009). Hydrodynamic impact of particle shape in slurry packed liquid chromatography columns. Journal of Chromatography A, 1216(30), 5687-5695. https://dx.doi.org/10.1016/j.chroma.2009.05.090
MLA:
Lottes, Florian, et al. "Hydrodynamic impact of particle shape in slurry packed liquid chromatography columns." Journal of Chromatography A 1216.30 (2009): 5687-5695.
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