Yeast profilin complements profilin deficiency in transgenic tomato fruits and allows development of hypoallergenic tomato fruits

Le LQ, Mahler V, Scheurer S, Foetisch K, Braun Y, Weigand D, Enrique E, Lidholm J, Paulus K, Sonnewald S, Vieths S, Sonnewald U (2010)

Publication Status: Published

Publication Type: Journal article

Publication year: 2010

Journal

Publisher: FEDERATION AMER SOC EXP BIOL

Book Volume: 24

Pages Range: 4939-4947

Journal Issue: 12

DOI: 10.1096/fj.10-163063

Abstract

Gene silencing of Lyc e 1 leads to reduced allergenicity of tomato fruits but impaired growth of transgenic tomato plants. The aim of the study was to restore growth of Lyc e 1-deficient tomato plants while retaining reduced allergenicity by simultaneous complementation of profilin deficiency by expression of nonallergenic yeast profilin. Transgenic plants were generated and tested by RT-PCR and immunoblotting; allergenicity of yeast profilin and transgenic fruits was investigated by IgE binding, basophil activation, and skin-prick tests. Lyc e 1 content of transgenic tomato fruits was <5% of that of wild-type plants, causing significantly reduced IgE antibody binding. Simultaneous coexpression of yeast profilin restored growth and biomass production almost to wildtype levels. Yeast profilin, sharing 32.6% amino acid sequence identity with Lyc e 1, displayed low IgE-binding capacity and allergenic potency. Among 16 tomato-allergic patients preselected for sensitization to Lyc e 1, none showed significant reactivity to yeast profilin. Yeast profilin did not induce mediator release, and coexpression of yeast profilin did not enhance the allergenicity of Lyc e 1-reduced fruits. Simultanous coexpression of yeast profilin allows silencing of tomato profilin and generation of viable plants with Lyc e 1-deficient tomato fruits. Therefore, a novel approach to allergen avoidance, genetically modified foods with reduced allergen accumulation, can be generated even if the allergen fulfills an essential cellular function in the plant. In summary, our findings of efficiently complementing profilin-deficient tomato plants by coexpression of low allergenic yeast profilin demonstrate the feasibility of creating low-allergenic food even if the allergen fulfills essential cellular functions.-Le, L. Q., Mahler, V., Scheurer, S., Foetisch, K., Braun, Y., Weigand, D., Enrique, E., Lidholm, J., Paulus, K. E., Sonnewald, S., Vieths, S., Sonnewald, U. Yeast profilin complements profilin deficiency in transgenic tomato fruits and allows development of hypoallergenic tomato fruits. FASEB J. 24, 4939-4947 (2010). www.fasebj.org

Authors with CRIS profile

Involved external institutions

Paul Ehrlich Institute / Paul-Ehrlich-Institut – Bundesinstitut für Impfstoffe und biomedizinische Arzneimittel (PEI) Germany (DE) Phadia AB Sweden (SE) Hospital General Universitari de Castelló Spain (ES)

How to cite

APA:

Le, L.Q., Mahler, V., Scheurer, S., Foetisch, K., Braun, Y., Weigand, D.,... Sonnewald, U. (2010). Yeast profilin complements profilin deficiency in transgenic tomato fruits and allows development of hypoallergenic tomato fruits. The FASEB Journal, 24(12), 4939-4947. https://doi.org/10.1096/fj.10-163063

MLA:

Le, Lien Q., et al. "Yeast profilin complements profilin deficiency in transgenic tomato fruits and allows development of hypoallergenic tomato fruits." The FASEB Journal 24.12 (2010): 4939-4947.

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