Systems Analysis of a Maize Leaf Developmental Gradient Redefines the Current C-4 Model and Provides Candidates for Regulation

Pick TR, Braeutigam A, Schlüter U, Denton AK, Colmsee C, Scholz U, Fahnenstich H, Pieruschka R, Rascher U, Sonnewald U, Weber APM (2011)

Publication Status: Published

Publication Type: Journal article

Publication year: 2011

Journal

The Plant Cell American Society of Plant Biologists

Publisher: AMER SOC PLANT BIOLOGISTS

Book Volume: 23

Pages Range: 4208-4220

Journal Issue: 12

DOI: 10.1105/tpc.111.090324

Abstract

We systematically analyzed a developmental gradient of the third maize (Zea mays) leaf from the point of emergence into the light to the tip in 10 continuous leaf slices to study organ development and physiological and biochemical functions. Transcriptome analysis, oxygen sensitivity of photosynthesis, and photosynthetic rate measurements showed that the maize leaf undergoes a sink-to-source transition without an intermediate phase of C-3 photosynthesis or operation of a photorespiratory carbon pump. Metabolome and transcriptome analysis, chlorophyll and protein measurements, as well as dry weight determination, showed continuous gradients for all analyzed items. The absence of binary on-off switches and regulons pointed to a morphogradient along the leaf as the determining factor of developmental stage. Analysis of transcription factors for differential expression along the leaf gradient defined a list of putative regulators orchestrating the sink-to-source transition and establishment of C-4 photosynthesis. Finally, transcriptome and metabolome analysis, as well as enzyme activity measurements, and absolute quantification of selected metabolites revised the current model of maize C-4 photosynthesis. All data sets are included within the publication to serve as a resource for maize leaf systems biology.

Authors with CRIS profile

Urte Schlüter Lehrstuhl für Biochemie Uwe Sonnewald Lehrstuhl für Biochemie

Involved external institutions

Heinrich-Heine-Universität Düsseldorf DE Germany (DE) Forschungszentrum Jülich / Research Centre Jülich (FZJ) DE Germany (DE) Metanomics Health GmbH DE Germany (DE) Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) / Leibniz Institute of Plant Genetics and Crop Plant Research DE Germany (DE)

How to cite

APA:

Pick, T.R., Braeutigam, A., Schlüter, U., Denton, A.K., Colmsee, C., Scholz, U.,... Weber, A.P.M. (2011). Systems Analysis of a Maize Leaf Developmental Gradient Redefines the Current C-4 Model and Provides Candidates for Regulation. The Plant Cell, 23(12), 4208-4220. https://doi.org/10.1105/tpc.111.090324

MLA:

Pick, Thea R., et al. "Systems Analysis of a Maize Leaf Developmental Gradient Redefines the Current C-4 Model and Provides Candidates for Regulation." The Plant Cell 23.12 (2011): 4208-4220.

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