Impaired excitability of renal afferent innervation after exposure to the inflammatory chemokine CXCL1

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autor(en): Ditting T, Freisinger W, Rodionova K, Schatz J, Lale N, Heinlein S, Linz P, Ott C, Schmieder R, Scrogin KE, Veelken R
Zeitschrift: American Journal of Physiology-Renal Physiology
Jahr der Veröffentlichung: 2016
Band: 310
Heftnummer: 5
Seitenbereich: F364-71
ISSN: 1931-857X


Abstract


Recently, we showed that renal afferent neurons exhibit a unique firing pattern, i.e., predominantly sustained firing, upon stimulation. Pathological conditions such as renal inflammation likely alter excitability of renal afferent neurons. Here, we tested whether the proinflammatory chemokine CXCL1 alters the firing pattern of renal afferent neurons. Rat dorsal root ganglion neurons (Th11-L2), retrogradely labeled with dicarbocyanine dye, were incubated with CXCL1 (20 h) or vehicle before patch-clamp recording. The firing pattern of neurons was characterized as tonic, i.e., sustained action potential (AP) firing, or phasic, i.e., <5 APs following current injection. Of the labeled renal afferents treated with vehicle, 58.9% exhibited a tonic firing pattern vs. 7.8%, in unlabeled, nonrenal neurons (P < 0.05). However, after exposure to CXCL1, significantly more phasic neurons were found among labeled renal neurons; hence the occurrence of tonic neurons with sustained firing upon electrical stimulation decreased (35.6 vs. 58.9%, P < 0.05). The firing frequency among tonic neurons was not statistically different between control and CXCL1-treated neurons. However, the lower firing frequency of phasic neurons was even further decreased with CXCL1 exposure [control: 1 AP/600 ms (1-2) vs. CXCL1: 1 AP/600 ms (1-1); P < 0.05; median (25th-75th percentile)]. Hence, CXCL1 shifted the firing pattern of renal afferents from a predominantly tonic to a more phasic firing pattern, suggesting that CXCL1 reduced the sensitivity of renal afferent units upon stimulation.



FAU-Autoren / FAU-Herausgeber

Lale, Nena
Medizinische Fakultät
Linz, Peter Dr. rer. nat.
Interdisziplinäres Zentrum für Klinische Forschung IZKF Prof.Dr.Kalden Medizinische Klinik 3
Ott, Christian PD Dr.
Medizinische Fakultät
Rodionova, Kristina Dr. med.
Medizinische Fakultät
Schatz, Johannes
Medizinische Fakultät
Schmieder, Roland Prof. Dr.
Professur für Innere Medizin (Nephrologie)
Veelken, Roland Prof. Dr.
Medizinische Fakultät


Autor(en) der externen Einrichtung(en)
Johannes Gutenberg-Universität Mainz
Loyola University Chicago


Zitierweisen

APA:
Ditting, T., Freisinger, W., Rodionova, K., Schatz, J., Lale, N., Heinlein, S.,... Veelken, R. (2016). Impaired excitability of renal afferent innervation after exposure to the inflammatory chemokine CXCL1. American Journal of Physiology-Renal Physiology, 310(5), F364-71. https://dx.doi.org/10.1152/ajprenal.00189.2015

MLA:
Ditting, Tilmann, et al. "Impaired excitability of renal afferent innervation after exposure to the inflammatory chemokine CXCL1." American Journal of Physiology-Renal Physiology 310.5 (2016): F364-71.

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Zuletzt aktualisiert 2018-08-10 um 02:23