Eisenried A, Wehrfritz A, Ihmsen H, Schüttler J, Jeleazcov C (2016)
Publication Type: Journal article
Publication year: 2016
Book Volume: 126
Pages Range: 148-55
DOI: 10.1016/j.jpba.2016.04.026
For the quantification of propofol total and unbound drug concentrations a sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated. To separate unbound propofol an ultrafiltration step before sample preparation was performed. Both the ultrafiltrate and plasma samples were extracted with solid-phase extraction and substituted with deuterated propofol as an internal standard. Separation was performed by gradient elution using UPLC-like system and analyzed by MS/MS consisting of an electrospray ionization source. To detect low and high concentration levels of propofol two calibration curves were identified and showed linearity within the range of 1-50ng/ml and 50-20000ng/ml. The lower limit of quantification was 1ng/ml. Intra- and interassay precision and accuracy did not exceed ±15%. The method was applied to a clinical study during intensive care treatment of patients after coronary artery bypass grafting.
APA:
Eisenried, A., Wehrfritz, A., Ihmsen, H., Schüttler, J., & Jeleazcov, C. (2016). Determination of total and unbound propofol in patients during intensive care sedation by ultrafiltration and LC-MS/MS. Journal of Pharmaceutical and Biomedical Analysis, 126, 148-55. https://doi.org/10.1016/j.jpba.2016.04.026
MLA:
Eisenried, Andreas, et al. "Determination of total and unbound propofol in patients during intensive care sedation by ultrafiltration and LC-MS/MS." Journal of Pharmaceutical and Biomedical Analysis 126 (2016): 148-55.
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