Magnetic fingerprints of rolling cells for quantitative flow cytometry in whole blood.

Reisbeck M, Helou MJ, Richter L, Kappes B, Friedrich O, Hayden O (2016)

Publication Language: English

Publication Status: Published

Publication Type: Journal article

Publication year: 2016

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 6

Pages Range: 32838

DOI: 10.1038/srep32838

Abstract

Over the past 50 years, flow cytometry has had a profound impact on preclinical and clinical applications requiring single cell function information for counting, sub-typing and quantification of epitope expression. At the same time, the workflow complexity and high costs of such optical systems still limit flow cytometry applications to specialized laboratories. Here, we present a quantitative magnetic flow cytometer that incorporates in situ magnetophoretic cell focusing for highly accurate and reproducible rolling of the cellular targets over giant magnetoresistance sensing elements. Time-of-flight analysis is used to unveil quantitative single cell information contained in its magnetic fingerprint. Furthermore, we used erythrocytes as a biological model to validate our methodology with respect to precise analysis of the hydrodynamic cell diameter, quantification of binding capacity of immunomagnetic labels, and discrimination of cell morphology. The extracted time-of-flight information should enable point-of-care quantitative flow cytometry in whole blood for clinical applications, such as immunology and primary hemostasis.

Authors with CRIS profile

Bärbel Kappes Lehrstuhl für Medizinische Biotechnologie Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie

Involved external institutions

Siemens AG, Healthcare Sector DE Germany (DE)

How to cite

APA:

Reisbeck, M., Helou, M.J., Richter, L., Kappes, B., Friedrich, O., & Hayden, O. (2016). Magnetic fingerprints of rolling cells for quantitative flow cytometry in whole blood. Scientific Reports, 6, 32838. https://dx.doi.org/10.1038/srep32838

MLA:

Reisbeck, Mathias, et al. "Magnetic fingerprints of rolling cells for quantitative flow cytometry in whole blood." Scientific Reports 6 (2016): 32838.

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