Complex reinnervation pattern after unilateral renal denervation in rats

Rodionova K, Fiedler C, Günther F, Grouzmann E, Neuhuber W, Fischer M, Ott C, Linz P, Freisinger W, Heinlein S, Schmidt S, Schmieder R, Amann KU, Scrogin K, Veelken R, Ditting T (2016)

Publication Type: Journal article

Publication year: 2016

Journal

American Journal of Physiology-Regulatory Integrative and Comparative Physiology American Physiological Society

Book Volume: 310

Pages Range: R806-18

Journal Issue: 9

DOI: 10.1152/ajpregu.00227.2014

Abstract

Renal denervation (DNX) is a treatment for resistant arterial hypertension. Efferent sympathetic nerves regrow, but reinnervation by renal afferent nerves has only recently been shown in the renal pelvis of rats after unilateral DNX. We examined intrarenal perivascular afferent and sympathetic efferent nerves after unilateral surgical DNX. Tyrosine hydroxylase (TH), CGRP, and smooth muscle actin were identified in kidney sections from 12 Sprague-Dawley rats, to distinguish afferents, efferents, and vasculature. DNX kidneys and nondenervated kidneys were examined 1, 4, and 12 wk after DNX. Tissue levels of CGRP and norepinephrine (NE) were measured with ELISA and mass spectrometry, respectively. DNX decreased TH and CGRP labeling by 90% and 95%, respectively (P < 0.05) within 1 wk. After 12 wk TH and CGRP labeling returned to baseline with a shift toward afferent innervation (P < 0.05). Nondenervated kidneys showed a doubling of both labels within 12 wk (P < 0.05). CGRP content decreased by 72% [3.2 ± 0.3 vs. 0.9 ± 0.2 ng/gkidney; P < 0.05] and NA by 78% [1.1 ± 0.1 vs. 0.2 ± 0.1 pmol/mgkidney; P < 0.05] 1 wk after DNX. After 12 wk, CGRP, but not NE, content in DNX kidneys was fully recovered, with no changes in the nondenervated kidneys. The use of phenol in the DNX procedure did not influence this result. We found morphological reinnervation and transmitter recovery of afferents within 12 wk after DNX. Despite morphological evidence of sympathetic regrowth, NE content did not fully recover. These results suggest a long-term net surplus of afferent influence on the DNX kidney may be contributing to the blood pressure lowering effect of DNX.

Authors with CRIS profile

Kristina Rodionova Medizinische Fakultät Christian Fiedler Medizinische Fakultät Franziska Günther Lehrstuhl für Physiologie Winfried Neuhuber Lehrstuhl für Mikroskopische Anatomie und Molekulare Zellbiologie Michael Fischer Lehrstuhl für Physiologie Christian Ott Medizinische Fakultät Peter Linz Interdisziplinäres Zentrum für Klinische Forschung IZKF-Förderlinien Roland Schmieder Professur für Innere Medizin (Nephrologie) Kerstin Ute Amann Department of Nephropathology Roland Veelken Medizinische Fakultät

Involved external institutions

Lausanne University Hospital / Centre hospitalier universitaire vaudois (CHUV) CH Switzerland (CH) Loyola University Chicago US United States (USA) (US)

How to cite

APA:

Rodionova, K., Fiedler, C., Günther, F., Grouzmann, E., Neuhuber, W., Fischer, M.,... Ditting, T. (2016). Complex reinnervation pattern after unilateral renal denervation in rats. American Journal of Physiology-Regulatory Integrative and Comparative Physiology, 310(9), R806-18. https://dx.doi.org/10.1152/ajpregu.00227.2014

MLA:

Rodionova, Kristina, et al. "Complex reinnervation pattern after unilateral renal denervation in rats." American Journal of Physiology-Regulatory Integrative and Comparative Physiology 310.9 (2016): R806-18.

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