Presence and distribution of L-kynurenine aminotransferases immunoreactivity in human cataractous lenses

Journal article


Publication Details

Author(s): Rejdak R, Oleszczuk A, Rummelt C, Turski WA, Choragiewicz T, Nowomiejska K, Ksiazek K, Thaler S, Zarnowski T, Okuno E, Grieb P, Zrenner E, Kruse F, Junemann AGM
Journal: Acta Ophthalmologica
Publication year: 2013
Volume: 91
Journal issue: 6
Pages range: e450-5
ISSN: 1755-375X


Abstract


To investigate the presence and distribution of l-kynurenine aminotransferases immunoreactivity in human and animal lenses during cataract formation.Immunohistochemistry was conducted using polyclonal antibodies against KAT I, KAT II and KAT III on sections of 26 anterior capsules from patients undergoing surgical treatment of anterior subcapsular cataract (ASC) and 22 cataractous lenses from human eyes enucleated because of choroidal malignant melanoma. Additionally, the eyes of 11-month-old DBA/2J mice (6 eyes) were investigated (with KAT I and II). Ten clear human lenses and four BL6 mice lenses were used as controls. Spatial immunoreactivity patterns of enzymes were compared with Periodic Acid - Schiff (PAS)-stained sections.Immunohistochemical analysis revealed presence of KAT I, KAT II and KAT III in extracellular structures of all studied types of cataract in human eyes showing specific pattern of the stain. In cortical cataract, immunoreactivity was observed on cortical lens fibres. In nuclear cataract, KAT II revealed stronger and diffused staining than KAT I. Additionally, both KAT showed more pronounced staining at the edge of small clefts. In normal human lenses, KAT I, II and III, immunoreactivity was not observed. Presence of KAT I and KAT II in the intercellular substance of DBA/2J mice cataract was observed. In BL6 mice lenses without cataract, only weak KAT I and KAT II staining was observed.Presence of l-kynurenine aminotransferases in extracellular matrix (ECM) during human cataract formation suggests that products of l-kynurenine pathway might be involved in mechanisms of cataractogenesis.



FAU Authors / FAU Editors

Kruse, Friedrich Prof. Dr.
Lehrstuhl für Augenheilkunde


External institutions with authors

Kyushu Nutrition Welfare University
Medical University of Lublin / Uniwersytet Medyczny w Lublinie
Polska Akademia Nauk (PAN) / Polish Academy of Sciences
Universitätsklinikum Tübingen


How to cite

APA:
Rejdak, R., Oleszczuk, A., Rummelt, C., Turski, W.A., Choragiewicz, T., Nowomiejska, K.,... Junemann, A.G.M. (2013). Presence and distribution of L-kynurenine aminotransferases immunoreactivity in human cataractous lenses. Acta Ophthalmologica, 91(6), e450-5. https://dx.doi.org/10.1111/aos.12138

MLA:
Rejdak, Robert, et al. "Presence and distribution of L-kynurenine aminotransferases immunoreactivity in human cataractous lenses." Acta Ophthalmologica 91.6 (2013): e450-5.

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Last updated on 2018-07-10 at 03:09