Schlötzer-Schrehardt U (2013)
Publication Type: Journal article
Publication year: 2013
Book Volume: 1014
Pages Range: 55-64
DOI: 10.1007/978-1-62703-432-6_3
While convincing data clearly suggest the presence of stem cells in the basal limbal epithelium in vivo, testing the proliferation, self-renewal, and differentiation capacity of stem cells relies on the development of methodologies that allow for their isolation and extensive propagation in vitro. Clonal analysis involving differentiation between short-lived transient cell clones and long-lived stem cell clones is an invaluable technique to identify stem cells in vitro, and allows cells to be expanded over multiple passages. This chapter describes a protocol for the isolation, expansion, and clonal analysis of limbal epithelial stem cells. The cultivation method described may be essential for long-term restoration of the damaged ocular surface in patients with limbal stem cell deficiency.
APA:
Schlötzer-Schrehardt, U. (2013). Clonal analysis of limbal epithelial stem cell populations. Methods in Molecular Biology, 1014, 55-64. https://dx.doi.org/10.1007/978-1-62703-432-6_3
MLA:
Schlötzer-Schrehardt, Ursula. "Clonal analysis of limbal epithelial stem cell populations." Methods in Molecular Biology 1014 (2013): 55-64.
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