Clonal analysis of limbal epithelial stem cell populations

Beitrag in einer Fachzeitschrift

Details zur Publikation

Autorinnen und Autoren: Schlötzer-Schrehardt U
Zeitschrift: Methods in molecular biology (Clifton, N.J.)
Jahr der Veröffentlichung: 2013
Band: 1014
Seitenbereich: 55-64
ISSN: 1064-3745


While convincing data clearly suggest the presence of stem cells in the basal limbal epithelium in vivo, testing the proliferation, self-renewal, and differentiation capacity of stem cells relies on the development of methodologies that allow for their isolation and extensive propagation in vitro. Clonal analysis involving differentiation between short-lived transient cell clones and long-lived stem cell clones is an invaluable technique to identify stem cells in vitro, and allows cells to be expanded over multiple passages. This chapter describes a protocol for the isolation, expansion, and clonal analysis of limbal epithelial stem cells. The cultivation method described may be essential for long-term restoration of the damaged ocular surface in patients with limbal stem cell deficiency.

FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Schlötzer-Schrehardt, Ursula apl. Prof. Dr.
Medizinische Fakultät

Zusätzliche Organisationseinheit(en)


Schlötzer-Schrehardt, U. (2013). Clonal analysis of limbal epithelial stem cell populations. Methods in molecular biology (Clifton, N.J.), 1014, 55-64.

Schlötzer-Schrehardt, Ursula. "Clonal analysis of limbal epithelial stem cell populations." Methods in molecular biology (Clifton, N.J.) 1014 (2013): 55-64.


Zuletzt aktualisiert 2018-07-10 um 03:08