Mapping the glycoxidation product Nepsilon-carboxymethyllysine in the milk proteome

Journal article


Publication Details

Author(s): Meyer B, Al-Diab D, Vollmer G, Pischetsrieder M
Journal: Proteomics
Publisher: Wiley-VCH Verlag
Publication year: 2011
Volume: 11
Pages range: 420-428
ISSN: 1615-9853


Abstract


Milk processing leads to severe protein damage caused by the formation of nonenzymatic

posttranslational modifications (nePTMs), such as glycation and glycoxidation. As a result, the technological and nutritional function of milk proteins can be critically altered. The present study investigated the protein-specific distribution of the glycoxidation product Ne-carboxymethyllysine (CML) in the proteome of processed milk. For this purpose, raw milk and heated milk were separated by 1-D or 2-DE. The distribution of CML in the milk proteome was examined by immunoblotting. The changes in the protein composition that occurred during heating were monitored by Coomassie staining. Relative modification rates were measured for the major milk protein fractions after 30 and 60 min of heating at 1201C and normalized to the content of the respective protein fraction in the samples. The highest glycoxidation rates were detected in the high molecular weight aggregates that are generated during heating. The casein fraction and the whey protein b-lactoglobulin were affected in a similar manner. The relevance of the results for industrial milk processing was confirmed by analyzing several commercial milk products accordingly. The presented approach allows nonenzymatic posttranslational modification mapping of the entire milk proteome.



FAU Authors / FAU Editors

Pischetsrieder, Monika Prof. Dr.
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)
Vollmer, Gregor
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)


How to cite

APA:
Meyer, B., Al-Diab, D., Vollmer, G., & Pischetsrieder, M. (2011). Mapping the glycoxidation product Nepsilon-carboxymethyllysine in the milk proteome. Proteomics, 11, 420-428. https://dx.doi.org/10.1002/pmic.201000233

MLA:
Meyer, Bianca, et al. "Mapping the glycoxidation product Nepsilon-carboxymethyllysine in the milk proteome." Proteomics 11 (2011): 420-428.

BibTeX: 

Last updated on 2018-19-06 at 15:23