Selection and characterization of chlorella mutants deficient in amino Acid transport : further evidence for three independent systems.

Beitrag in einer Fachzeitschrift
(Originalarbeit)


Details zur Publikation

Autorinnen und Autoren: Sauer N, Tanner W
Zeitschrift: Plant Physiology
Jahr der Veröffentlichung: 1985
Band: 79
Heftnummer: 3
Seitenbereich: 760-764
ISSN: 0032-0889
Sprache: Englisch


Abstract


Six amino acids are transported at high rates across the plasmalemma of Chlorella vulgaris only after the induction of two specific transport systems. Induction is achieved either by pretreatment with glucose, glucose analogs, or by nitrogen starvation. Mutants for these transport systems were obtained after incubation of Chlorella cells in the presence of acridine orange or ethidium bromide, followed by a selection procedure using the toxic amino acid analogs l-canavanine (for l-arginine), and l-azetidine-2-carboxylic acid (for l-proline). Mutants isolated by this method had lost their ability to induce the corresponding transport system. Double mutants deficient in transport of both these amino acids still possess the general amino acid transport system, a third system which was described previously. Evidence for additional amino acid transport systems in Chlorella is discussed.



FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Sauer, Norbert Prof. Dr.
Lehrstuhl für Molekulare Pflanzenphysiologie


Einrichtungen weiterer Autorinnen und Autoren

Universität Regensburg


Zitierweisen

APA:
Sauer, N., & Tanner, W. (1985). Selection and characterization of chlorella mutants deficient in amino Acid transport : further evidence for three independent systems. Plant Physiology, 79(3), 760-764. https://dx.doi.org/10.1104/pp.79.3.760

MLA:
Sauer, Norbert, and Widmar Tanner. "Selection and characterization of chlorella mutants deficient in amino Acid transport : further evidence for three independent systems." Plant Physiology 79.3 (1985): 760-764.

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