Selection and characterization of chlorella mutants deficient in amino Acid transport : further evidence for three independent systems.

Journal article
(Original article)


Publication Details

Author(s): Sauer N, Tanner W
Journal: Plant Physiology
Publication year: 1985
Volume: 79
Journal issue: 3
Pages range: 760-764
ISSN: 0032-0889
Language: English


Abstract


Six amino acids are transported at high rates across the plasmalemma of Chlorella vulgaris only after the induction of two specific transport systems. Induction is achieved either by pretreatment with glucose, glucose analogs, or by nitrogen starvation. Mutants for these transport systems were obtained after incubation of Chlorella cells in the presence of acridine orange or ethidium bromide, followed by a selection procedure using the toxic amino acid analogs l-canavanine (for l-arginine), and l-azetidine-2-carboxylic acid (for l-proline). Mutants isolated by this method had lost their ability to induce the corresponding transport system. Double mutants deficient in transport of both these amino acids still possess the general amino acid transport system, a third system which was described previously. Evidence for additional amino acid transport systems in Chlorella is discussed.



FAU Authors / FAU Editors

Sauer, Norbert Prof. Dr.
Lehrstuhl für Molekulare Pflanzenphysiologie


External institutions with authors

Universität Regensburg


How to cite

APA:
Sauer, N., & Tanner, W. (1985). Selection and characterization of chlorella mutants deficient in amino Acid transport : further evidence for three independent systems. Plant Physiology, 79(3), 760-764. https://dx.doi.org/10.1104/pp.79.3.760

MLA:
Sauer, Norbert, and Widmar Tanner. "Selection and characterization of chlorella mutants deficient in amino Acid transport : further evidence for three independent systems." Plant Physiology 79.3 (1985): 760-764.

BibTeX: 

Last updated on 2018-01-07 at 02:10