Selection and characterization of chlorella mutants deficient in amino Acid transport : further evidence for three independent systems.
Sauer N, Tanner W (1985)
Publication Language: English
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 1985
Journal
Book Volume: 79
Pages Range: 760-764
Journal Issue: 3
DOI: 10.1104/pp.79.3.760
Abstract
Six amino acids are transported at high rates across the plasmalemma of Chlorella vulgaris only after the induction of two specific transport systems. Induction is achieved either by pretreatment with glucose, glucose analogs, or by nitrogen starvation. Mutants for these transport systems were obtained after incubation of Chlorella cells in the presence of acridine orange or ethidium bromide, followed by a selection procedure using the toxic amino acid analogs l-canavanine (for l-arginine), and l-azetidine-2-carboxylic acid (for l-proline). Mutants isolated by this method had lost their ability to induce the corresponding transport system. Double mutants deficient in transport of both these amino acids still possess the general amino acid transport system, a third system which was described previously. Evidence for additional amino acid transport systems in Chlorella is discussed.
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Germany (DE)How to cite
APA:
Sauer, N., & Tanner, W. (1985). Selection and characterization of chlorella mutants deficient in amino Acid transport : further evidence for three independent systems. Plant Physiology, 79(3), 760-764. https://dx.doi.org/10.1104/pp.79.3.760
MLA:
Sauer, Norbert, and Widmar Tanner. "Selection and characterization of chlorella mutants deficient in amino Acid transport : further evidence for three independent systems." Plant Physiology 79.3 (1985): 760-764.
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