Influence of different irradiation protocols on vascularization and bone formation parameters in rat femora.

Journal article


Publication Details

Author(s): Rottensteiner-Brandl U, Distel L, Stumpf M, Fey T, Köhn K, Bertram U, Lingens LF, Greil P, Horch R, Arkudas A
Journal: Tissue Engineering - Part C: Methods
Publication year: 2017
ISSN: 1937-3392


Abstract


INTRODUCTION Aim of the present study was the establishment of an efficient and reproducible model for irradiation of rat femora as a model for impaired osteogenesis and angiogenesis. MATERIALS AND METHODS Four different irradiation protocols were compared: single irradiation of the left femur with 20 Gy and explantation after 4 or 8 weeks (group A, B); three irradiation fractions at 3-4 days intervals with 10 Gy and explantation after 4 or 8 weeks (group C, D). The contralateral, unirradiated femur served as control. Evaluation included histology, microcomputertomography (μCT) and real time PCR.\nRESULTS\nHistology showed a pronounced increase of vacuoles in bone marrow after irradiation, especially after 4 weeks (group A and C), demonstrating bone marrow edema and fatty degeneration. Irradiation provoked a decrease of total cell numbers in cortical bone and of Hif1α positive cells in bone marrow. The expression of several markers (osteocalcin, RUNX2, TGFβ1, TNFα, VEGF-A, Hif1α) was decreased in group A after irradiation. This might suggest a decreased metabolism after irradiation. A significant decrease in small-sized vessels was seen in μCT evaluation in group A and D. CONCLUSIONS Single irradiation with 20 Gy had the most severe and reproducible impact on osteogenesis and angiogenesis after 4 weeks while being well tolerated by all animals, thus making it an excellent model for evaluation of bone healing and vascularization in irradiated tissue.



FAU Authors / FAU Editors

Arkudas, Andreas Prof. Dr.
Medizinische Fakultät
Plastisch- und Handchirurgische Klinik
Bertram, Ulf
Plastisch- und Handchirurgische Klinik
Distel, Luitpold Prof. Dr.
Medizinische Fakultät
Fey, Tobias Dr.
Lehrstuhl für Werkstoffwissenschaften (Glas und Keramik)
Greil, Peter Prof. Dr.
Lehrstuhl für Werkstoffwissenschaften (Glas und Keramik)
Horch, Raymund Prof. Dr.
Professur für Plastische Chirurgie und Handchirurgie
Köhn, Katrin
Rottensteiner-Brandl, Ulrike Dr.
Lehrstuhl für Biochemie und Molekulare Medizin
Stumpf, Martin
Lehrstuhl für Werkstoffwissenschaften (Glas und Keramik)


How to cite

APA:
Rottensteiner-Brandl, U., Distel, L., Stumpf, M., Fey, T., Köhn, K., Bertram, U.,... Arkudas, A. (2017). Influence of different irradiation protocols on vascularization and bone formation parameters in rat femora. Tissue Engineering - Part C: Methods. https://dx.doi.org/10.1089/ten.TEC.2017.0170

MLA:
Rottensteiner-Brandl, Ulrike, et al. "Influence of different irradiation protocols on vascularization and bone formation parameters in rat femora." Tissue Engineering - Part C: Methods (2017).

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Last updated on 2018-16-06 at 21:23