Maia de Pádua R, Meitinger N, de Souza Filho JD, Waibel R, Gmeiner P, Braga FC, Kreis W (2012)
Publication Language: English
Publication Type: Journal article, Original article
Publication year: 2012
Publisher: Elsevier
Book Volume: 77
Pages Range: 1373-1380
Journal Issue: 13
DOI: 10.1016/j.steroids.2012.07.016
Cell cultures of Digitalis species are known to accept exogenous substrates for biotransformation reactions. We here report the biotransformation of 21-O-acetyl-deoxycorticosterone (1) by cell suspension cultures of Digitalis lanata strain W.1.4. Nine derivatives of 1 were obtained and their chemical structures determined by spectroscopic methods. 2β-Hydroxylation and C-21-glucosylation of the steroidal nucleus were described for the first time in suspension-cultured plant cells. Steroid 5α- and 5β-reduction products were also observed. Among the compounds isolated and structures elucidated were 2β,3β,21-trihydroxy-4-pregnen-20-one, 2β,3α,21-trihydroxy-4-pregnen-20-one and 3β,21-dihydroxy- 5α-pregnan-20-one-3β-O-β-glucoside. © 2012 Elsevier Inc. All rights reserved.
APA:
Maia de Pádua, R., Meitinger, N., de Souza Filho, J.D., Waibel, R., Gmeiner, P., Braga, F.C., & Kreis, W. (2012). Biotransformation of 21-O-acetyl-deoxycorticosterone by cell suspension cultures of Digitalis lanata (strain W.1.4). Steroids, 77(13), 1373-1380. https://dx.doi.org/10.1016/j.steroids.2012.07.016
MLA:
Maia de Pádua, Rodrigo, et al. "Biotransformation of 21-O-acetyl-deoxycorticosterone by cell suspension cultures of Digitalis lanata (strain W.1.4)." Steroids 77.13 (2012): 1373-1380.
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