Full Length Interleukin 33 Aggravates Radiation-Induced Skin Reaction

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autorinnen und Autoren: Kurow O, Frey B, Schuster L, Schmitt V, Adam S, Hahn M, Gilchrist D, Mcinnes IB, Wirtz S, Gaipl U, Krönke G, Schett G, Frey S, Hueber A
Zeitschrift: Frontiers in Immunology
Jahr der Veröffentlichung: 2017
Band: 8
Seitenbereich: 722
ISSN: 1664-3224


Abstract


The interleukin (IL)-1 family member IL-33 has been described as intracellular alarmin with broad roles in wound healing, skin inflammation but also autoimmunity. Its dichotomy between full length (fl) IL-33 and the mature (m) form of IL-33 and its release by necrosis is still not fully understood. Here, we compare functional consequences of both forms in the skin in vivo, and therefore generated two lines of transgenic mice which selectively overexpress mmIL-33 and flmIL-33 in basal keratinocytes. Transgene mRNA was expressed at high level in skin of both lines but not in organs due to the specific K14 promoter. We could demonstrate that transgenic overexpression of mmIL-33 in murine keratinocytes leads to a spontaneous skin inflammation as opposed to flmIL-33. K14-mmIL-33 mice synthesize and secrete high amounts of mmIL-33 along with massive cutaneous manifestations, like increased epidermis and dermis thickness, infiltration of mast cells in the epidermis and dermis layers and marked hyperkeratosis. Using skin inflammation models such as IL-23 administration, imiquimod treatment, or mechanical irritation did not lead to exacerbated inflammation in the K14-flmIL-33 strain. As radiation induces a strong dermatitis due to apoptosis and necrosis, we determined the effect of fractionated radiation (12 Gy, 4 times). In comparison to wild-type mice, an increase in ear thickness in flmIL-33 transgenic mice was observed 25 days after irradiation. Macroscopic examination showed more severe skin symptoms in irradiated ears compared to controls. In summary, secreted mmIL-33 itself has a potent capacity in skin inflammation whereas fl IL-33 is limited due to its intracellular retention. During tissue damage, fl IL-33 exacerbated radiation-induced skin reaction.



FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Adam, Susanne
Medizinische Klinik 3 - Rheumatologie und Immunologie
Frey, Silke Dr. rer. nat.
Medizinische Klinik 3 - Rheumatologie und Immunologie
Frey, Benjamin PD Dr.
Strahlenklinik
Gaipl, Udo Prof. Dr.
Strahlenklinik
Hueber, Axel PD Dr.
Medizinische Klinik 3 - Rheumatologie und Immunologie
Krönke, Gerhard Prof. Dr. med.
Medizinische Klinik 3 - Rheumatologie und Immunologie
Schett, Georg Prof. Dr. med.
Lehrstuhl für Innere Medizin III
Schmitt, Verena
Professur für Experimentelle Immuntherapie
Wirtz, Stefan PD Dr.
Medizinische Klinik 1 - Gastroenterologie, Pneumologie und Endokrinologie


Einrichtungen weiterer Autorinnen und Autoren

University of Glasgow


Zitierweisen

APA:
Kurow, O., Frey, B., Schuster, L., Schmitt, V., Adam, S., Hahn, M.,... Hueber, A. (2017). Full Length Interleukin 33 Aggravates Radiation-Induced Skin Reaction. Frontiers in Immunology, 8, 722. https://dx.doi.org/10.3389/fimmu.2017.00722

MLA:
Kurow, Olga, et al. "Full Length Interleukin 33 Aggravates Radiation-Induced Skin Reaction." Frontiers in Immunology 8 (2017): 722.

BibTeX: 

Zuletzt aktualisiert 2019-18-07 um 07:32