?B-crystallin is a sensor for assembly intermediates and for the subunit topology of desmin intermediate filaments

Journal article


Publication Details

Author(s): Sharma S, Conover GM, Elliott JL, Perng MD, Herrmann H, Quinlan RA
Journal: Cell Stress & Chaperones
Publication year: 2017
Volume: 22
Journal issue: 4
Pages range: 613-626
ISSN: 1355-8145


Abstract

Mutations in the small heat shock protein chaperone CRYAB (?B-crystallin/HSPB5) and the intermediate filament protein desmin, phenocopy each other causing cardiomyopathies. Whilst the binding sites for desmin on CRYAB have been determined, desmin epitopes responsible for CRYAB binding and also the parameters that determine CRYAB binding to desmin filaments are unknown. Using a combination of co-sedimentation centrifugation, viscometric assays and electron microscopy of negatively stained filaments to analyse the in vitro assembly of desmin filaments, we show that the binding of CRYAB to desmin is subject to its assembly status, to the subunit organization within filaments formed and to the integrity of the C-terminal tail domain of desmin. Our in vitro studies using a rapid assembly protocol, C-terminally truncated desmin and two disease-causing mutants (I451M and R454W) suggest that CRYAB is a sensor for the surface topology of the desmin filament. Our data also suggest that CRYAB performs an assembly chaperone role because the assembling filaments have different CRYAB-binding properties during the maturation process. We suggest that the capability of CRYAB to distinguish between filaments with different surface topologies due either to mutation (R454W) or assembly protocol is important to understanding the pathomechanism(s) of desmin-CRYAB myopathies.


External institutions with authors

Deutsches Krebsforschungszentrum (DKFZ)
Durham University
National Tsing Hua University
Texas A&M University


How to cite

APA:
Sharma, S., Conover, G.M., Elliott, J.L., Perng, M.D., Herrmann, H., & Quinlan, R.A. (2017). ?B-crystallin is a sensor for assembly intermediates and for the subunit topology of desmin intermediate filaments. Cell Stress & Chaperones, 22(4), 613-626. https://dx.doi.org/10.1007/s12192-017-0788-7

MLA:
Sharma, Sarika, et al. "?B-crystallin is a sensor for assembly intermediates and for the subunit topology of desmin intermediate filaments." Cell Stress & Chaperones 22.4 (2017): 613-626.

BibTeX: 

Last updated on 2018-06-10 at 03:13