The effects of Kv1.3 and IKCa1 channel inhibition on cytokine production and calcium influx of T lymphocytes in rheumatoid arthritis and ankylosing spondylitis

Toldi G, Munoz LE, Herrmann M, Schett G, Balog A, Munoz Becerra L (2016)

Publication Type: Journal article, Original article

Publication year: 2016

Journal

Immunologic Research Humana Press

Book Volume: 64

Pages Range: 627-31

Journal Issue: 2

DOI: 10.1007/s12026-015-8683-8

Abstract

Kv1.3 and IKCa1 lymphocyte potassium channels have been implicated as important targets of selective immunomodulation. We compared the alterations in cytokine production upon selective inhibition of Kv1.3 or IKCa1 channels (by MGTX and TRAM, respectively) in healthy donors (HD), RA and AS patients. We also determined calcium influx kinetics and its sensitivity to Kv1.3 and IKCa1 channel inhibition following PHA activation in CD4, Th1, Th2 and CD8 cells as well as monocytes. The application of TRAM resulted in a lower production of TNF-a and IL1-RA in all three study groups. Inhibition by TRAM had contrary effects on the production of IL-1b and IL-5: While their production was increased by PBMCs of RA patients, this effect was not observed in HD and AS PBMCs. While treatment with MGTX resulted in a similar decrease in calcium influx in the CD4 and Th2 subsets across all study groups, TRAM treatment had opposite effects on RA and HD samples: It decreased calcium influx in the Th2 and CD8 subsets in RA, while only Th1 cells were affected in HDs. The effects of IKCa1 channel inhibition are controversial in samples of RA and AS patients, since it shifts the inflammatory balance into the pro-inflammatory direction.

Authors with CRIS profile

Luis Munoz Becerra Department of Medicine 3 – Rheumatology and Immunology Martin Herrmann Department of Medicine 3 – Rheumatology and Immunology Georg Schett Lehrstuhl für Innere Medizin III

Involved external institutions

University of Szeged / József Attila Tudományegyetem Szeged HU Hungary (HU) Semmelweis University / Semmelweis Egyetem HU Hungary (HU)

How to cite

APA:

Toldi, G., Munoz, L.E., Herrmann, M., Schett, G., Balog, A., & Munoz Becerra, L. (2016). The effects of Kv1.3 and IKCa1 channel inhibition on cytokine production and calcium influx of T lymphocytes in rheumatoid arthritis and ankylosing spondylitis. Immunologic Research, 64(2), 627-31. https://dx.doi.org/10.1007/s12026-015-8683-8

MLA:

Toldi, Gergely, et al. "The effects of Kv1.3 and IKCa1 channel inhibition on cytokine production and calcium influx of T lymphocytes in rheumatoid arthritis and ankylosing spondylitis." Immunologic Research 64.2 (2016): 627-31.

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