Zaloga J, Poettler M, Leitinger G, Friedrich RP, Almer G, Lyer S, Baum E, Tietze R, Heimke-Brinck R, Mangge H, Dörje F, Lee G, Alexiou C (2016)
Publication Type: Journal article
Publication year: 2016
Book Volume: 101
Pages Range: 152-62
DOI: 10.1016/j.ejpb.2016.01.017
In this work we present a new formulation of superparamagnetic iron oxide nanoparticles (SPIONs) for magnetic drug targeting. The particles were reproducibly synthesized from current good manufacturing practice (cGMP) - grade substances. They were surface coated using fatty acids as anchoring molecules for human serum albumin. We comprehensively characterized the physicochemical core-shell structure of the particles using sophisticated methods. We investigated biocompatibility and cellular uptake of the particles using an established flow cytometric method in combination with microwave-plasma assisted atomic emission spectroscopy (MP-AES). The cytotoxic drug mitoxantrone was adsorbed on the protein shell and we showed that even in complex media it is slowly released with a close to zero order kinetics. We also describe an in vitro proof-of-concept assay in which we clearly showed that local enrichment of this SPION-drug conjugate with a magnet allows site-specific therapeutic effects.
APA:
Zaloga, J., Poettler, M., Leitinger, G., Friedrich, R.P., Almer, G., Lyer, S.,... Alexiou, C. (2016). Pharmaceutical formulation of HSA hybrid coated iron oxide nanoparticles for magnetic drug targeting. European Journal of Pharmaceutics and Biopharmaceutics, 101, 152-62. https://dx.doi.org/10.1016/j.ejpb.2016.01.017
MLA:
Zaloga, Jan, et al. "Pharmaceutical formulation of HSA hybrid coated iron oxide nanoparticles for magnetic drug targeting." European Journal of Pharmaceutics and Biopharmaceutics 101 (2016): 152-62.
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