N2-(1-Carboxyethyl)deoxyguanosine, a nonenzymatic glycation adduct of DNA induces single strand breaks and increases mutation frequencies
Pischetsrieder M, Seidel W, Münch G, Schinzel R (1999)
Publication Type: Journal article
Publication year: 1999
Journal
Publisher: Elsevier
Book Volume: 264
Pages Range: 544-549
Abstract
N2-(1-Carboxyethyl)deoxyguanosine (CEdG) is a major nonenzymatic glycation product of DNA. The effect of CEdG modification, which was specifically prepared by incubation with dihydroxyacetone, on plasmid DNA topology was evaluated by gel electrophoresis. A time-dependent decrease of supercoiled plasmid-DNA was observed in parallel to the increase of CEdG adducts; the half-life time of the supercoiled plasmid-DNA was estimated to be approximately 16-18 h. CEdG-modified plasmid DNA showed a 25-fold re duced transformation efficiency. When modified DNA was used to transform Escherichia coli cells, a 6-fold increase in mutation frequency was determined by measuring loss of alpha-complementation. For the mutator strain BMH71-18mutS, an 8-fold increase in mutation frequency was observed. Although the exact mechanism of DNA damage is unclear, the occurrence of spontaneous depurination is likely. These findings suggest that a defined DNA glycation reaction can lead to DNA damage in vivo.
Authors with CRIS profile
Involved external institutions
Germany (DE)How to cite
APA:
Pischetsrieder, M., Seidel, W., Münch, G., & Schinzel, R. (1999). N2-(1-Carboxyethyl)deoxyguanosine, a nonenzymatic glycation adduct of DNA induces single strand breaks and increases mutation frequencies. Biochemical and Biophysical Research Communications, 264, 544-549. https://dx.doi.org/10.1006/bbrc.1999.1528
MLA:
Pischetsrieder, Monika, et al. "N2-(1-Carboxyethyl)deoxyguanosine, a nonenzymatic glycation adduct of DNA induces single strand breaks and increases mutation frequencies." Biochemical and Biophysical Research Communications 264 (1999): 544-549.
BibTeX: Download